Animal Genetics Breeding Metabolomics Services
The development of animal phenomics characterized by precision and high throughput as well as physiological genomics and physiological genetics research has forced the increased use of metabolomics techniques and methods in the study of resolving the genetic mechanisms of economically important traits in animals. Variations in the animal genome do not necessarily have a direct effect on the animal phenotype, but changes in the associated metabolic molecules may be the end result of that variation. Metabolomics data allows correlation analysis of phenotypic and genetic variants. Creative Proteomics provides a one-stop metabolomics solution for animal genetic breeding research.
Metabolomics analysis services can be applied to animal genetics breeding research:
- Estimation of molecular genetic parameters of animal metabolism: metabolic levels within an organism change over time and are more susceptible to environmental and lifestyle habits. The metabolomic profile of each biological sample simply portrays the metabolic state of that individual at a given time. When performing genetic analysis of metabolic molecule expression as an intermediate trait, the share of genetic factors explaining the phenotypic variation of these metabolic molecules needs to be fully understood.
- Analysis of metabolic profiling differences between breeds: In metabolomics studies of domesticated animals such as cattle, pigs and chickens, some metabolic molecules are screened as biomarkers to distinguish between breeds by testing animal serum, tissues, milk and other study materials.
- Metabolome genome-wide association analysis: The expression of metabolic molecules detected in serum, blood, urine and other body fluids are used as phenotypic values to carry out genome-wide association analysis of metabolic molecules.
- Screening for biomarkers affecting economically important traits: Correlation or difference analysis between metabolic molecules and economically important traits is studied to screen biomarkers. Using these screened metabolic molecules as biomarkers, they are applied in breeding marker-assisted selection, thus improving the accuracy of selection.
Types of Metabolites That Can be Analyzed:
|Amino acids||Small molecules involved in metabolic processes such as glycine, serine and threonine metabolism, alanine and aspartate metabolism, glutamate metabolism, histidine metabolism, lysine metabolism, phenylalanine and tyrosine metabolism, tryptophan metabolism, valine, leucine and isoleucine metabolism, cysteine, methionine and taurine metabolism, arginine-like and proline-like metabolism, and the urea cycle|
|Peptides||Small molecules involved in metabolic processes such as dipeptide and gamma-glutamyl|
|Carbohydrates||Small molecules involved in the metabolism of fructose, mannose, galactose, starch and sucrose, nucleotide sugars, pentose, and other substances, as well as in processes such as glycolysis, gluconeogenesis and pyruvate metabolism|
|Energy||Small molecules involved in tricarboxylic acid cycle, oxidative phosphorylation and other related metabolic processes|
|Lipids||Small molecules involved in the metabolism of essential fatty acids, medium-chain fatty acids, long-chain fatty acids, carnitine, bile acids, glycerides, lysophospholipids, monoacylglycerols, sphingolipids, and sterols/steroids|
|Nucleotides||Small molecules involved in metabolic processes related to purines or hypoxanthines or inosine, pyrimidines or thymidine, valine, leucine and isoleucine, etc.|
|Vitamins and cofactors||Small molecules involved in metabolic processes related to ascorbate and aldehyde, hemoglobin and porphyrins, pantothenic acid and coenzyme A and tocopherols|
|Exogenous compounds||Small molecules including exogenous chemical molecules and drugs|
The Process for Metabolomics Analysis Services
Technology Platform for Metabolomics Analysis
Multiple analytical platforms, including GC-MS, LC-MS, UPLC-MS.
|GC (Q Exactive) (- mode MS)|
HPLC: HILIC (+/- mode MS)
HPLC: RP C18 (+/-mode MS)
IC (- mode MS)
|Q Exactive MS|
Orbitrap Tribrid MS
Creative Proteomics is a good partner in the agriculture field and has extensive experience in metabolomic analysis. We provide metabolomics solutions for animal genetics breeding research to accelerate the exploration of new scientific horizons. If you would like to learn more, please contact us. We look forward to working with you.
For Research Use Only. Not for use in diagnostic procedures.