Central Carbon Metabolism Analysis

Central carbon metabolism (CCM) pathway related metabolites include dozens of metabolites in glucose involved tricarboxylic acid cycle (TCA), glycolytic pathway (EMP), pentose phosphate pathway (PPP) and other pathways.

Central carbon metabolism is the main source of energy required by living organisms and provides precursors for other metabolisms in the body. Redox reactions are one of the major chemical reactions and oxidoreductases are the enzymes responsible for catalysing these reactions. In metabolic pathways such as TCA and PPP, oxidoreductases play the role of key enzymes.

Glucose is the most important energy substance, performing physiological functions such as supplying energy for oxidation, providing the carbon source needed for lipid and protein synthesis, and forming glycolipids. Its breakdown in the organism is mainly through central carbon metabolism. When glucose metabolism is abnormal, it can cause various diseases in the body, such as diabetes and its complications. In addition, the development of diseases such as diabetes, atherosclerotic heart disease, abnormal glucose metabolism in pregnancy and liver disease are also closely related to abnormal glucose metabolism. Abnormalities in lactate and pyruvate are likely to be related to the glycolytic pathway. Some of the metabolic intermediates of other glycolytic pathways also provide precursors and raw materials for lipids, amino acids and some other biosynthesis. The predominantly anaerobic respiration of cells rather than normal aerobic oxidation is an important factor in identifying the presence of tumors.

The intermediates of the TCA cycle are used as substrates for the biosynthesis of amino acids, nucleic acids and cell wall components required for plant growth and development.

LC-QTRAP-MS allows for high throughput determination of substances in the central carbon metabolic pathway of a sample and data analysis to determine the relationships between substances. Creative Proteomics can realize quantitative and qualitative analysis of various substances involved in central carbon metabolism.

Service Workflow

Sample Requirements

• Plasma/serum ≥10 µL
• Tissues ≥10 mg
• Cell ≥1×10⁶
• Cell medium ≥ 100 µL

Please contact us for other sample types.

Deliverables

• Experimental procedure
• Parameters of LC and MS
• MS raw data files and MS data quality checks
• Metabolites quantification data
• Custom analysis report

Creative Proteomics offers several approaches to provide central carbon metabolism analysis service and deliver precise and detailed data and analysis report. If you want to detect other metabolites, you can tell us through the inquiry form, and our technicians will communicate with you.