Plant Targeted Metabolomics Service

Overview

Metabolomics is a comprehensive, unbiased, high-throughput scientific study and analysis of complex metabolite mixtures typical of plant extracts. The general analysis of metabolic composition requires multiple steps: sample extraction, metabolite isolation/assay/identification, automated data collection/processing/analysis and quantification.

Plant metabolomics enables the establishment of plant metabolic networks and the measurement of their responses to environmental and genetic changes, and also contributes insights into plant phenotypes to development, physiology, tissue properties, resistance, biodiversity, etc.

Targeted metabolomics provides a high-resolution tool for the identification and quantitative analysis of targeted metabolites in plants. It has the advantage of high specificity and accuracy and has been widely used to analyze and compare targeted metabolic compounds across different physiological states or developmental stages. Creative Proteomics offers a wide range of plant targeted metabolomics services covering hundreds of plant metabolites including phytohormones, carotenoids, chlorophylls, lectins, toxins and more.

The chemical composition in plants is complex, with a wide range of structures and diverse biological activities, such as nucleic acids, proteins, glycans, alkaloids, terpenoids, sterols, etc. Based on LC-MS/MS technology, phytochemical analysis can also be realized to provide technical support to solve the analysis in pharmacology (e.g. in vivo metabolic process analysis of drugs), environmental science (e.g. organic pollutant detection), food science (e.g. monitoring of various additives), agronomy (e.g. pesticide residue control, soil organic matter testing, hormone analysis), etc.

Advantages of Our Plant Targeted Metabolomics Service

Rich experience in plant sample handling and metabolite extraction.

Cutting edge MS platforms: LC-MS, GC-MS; MRM method

Quantitative analysis of targeted plant metabolites

Professional statistical analysis and bioinformatics analysis

Service Workflow

The plant targeted metabolomics service provided by Creative Proteomics is based on our cutting-edge chromatographic separation and mass spectrometry platforms. This diagram demonstrates the rough steps in plant targeted metabolomics studies. Our service will be tailored to specific samples and needs for optimal results.

List of Plant Targeted Metabolites Analysis Services

Sample Requirements

Plant tissues from leaf, flower, stem, root, seed, or fruit: 200 mg/sample. Samples should be frozen in liquid nitrogen immediately after sample collection, and then transferred to -80°C for storage.

Deliverables

• Experiment procedures
• Parameters of GC/LC and MS
• GC/LC and MS raw data files and MS data quality checks
• Metabolites quantification data
• Bioinformatics analysis report (PCA, KEGG, etc.)

Creative Proteomics has extensive experience in plant sample processing, small molecule compound extraction and performing custom bioinformatics analyses. If you want to detect other substances or learn more, please contact us, we are happy to serve you.

Reference

1. Majumdar, S., & Keller, A. A. (2021). Omics to address the opportunities and challenges of nanotechnology in agriculture. Critical Reviews in Environmental Science and Technology, 51(22), 2595-2636.