Peonidin Analysis Service

Targeted metabolome workflow of peonidin

Targeted metabolome studies can establish baseline metabolite levels in tissues or organisms for comparison among experiments under different conditions.

Design experiments and sample processing

Standard preparation and mass spectrometry detection，and make a standard curve

Extraction of metabolites in samples

Method verification and data quality control

Raw data preprocessing and absolute quantification of metabolites based on standard curve

Bioinformatics analysis (PCA analysis, cluster analysis, difference analysis, etc.)

Sample requirements

Sample type

If it is a cell sample, centrifuge after sampling, remove the medium, immediately put it in liquid nitrogen, and store it at -80℃.

If it is a tissue sample, please put it in liquid nitrogen immediately after separation and store it at -80℃.

Mixed sample

Each sample was mixed from more than 6 tissues under the same conditions and quickly put into liquid nitrogen.

※The same condition refers to the same period, basically the same phenotype, and the same part.

Sample weight and repeat

It is recommended that the total amount of fresh and clean plant samples is >1g, and need to prepare more than 6 biological replicates.

Storage and transportation

Dry ice transportation (dry ice volatilizes 3-4 kg/day, please use sufficient dry ice for transportation).

※The experimental samples will not be returned, please back them up by yourself.

Service process

Delivery standard

• Experimental procedure
• Parameters of HPLC and MS
• Raw data, chromatograms and mass spectra
• Metabolites quantification data
• Custom analysis report

Project cycle

About 1-3 weeks

Creative Proteomics has leading technology, first-class R&D and production equipment, and has established a complete metabolome service system. Our professional team has many years of experience in metabolomics, bioinformatics, and statistics, and can help you plan, conduct and report metabolomics research, so that your upstream and downstream experiments can be seamlessly connected. We can provide a wide range of target metabolomics services, including target metabolic pathways, target metabolite analysis, etc. You can also perform personalized customization services at the same time during the data analysis stage. If you have any questions or specific requirements, please feel free to contact us.

References

1. Akhatou I, Sayago A, et al. Application of targeted metabolomics to investigate optimum growing conditions to enhance bioactive content of strawberry. Journal of agricultural and food chemistry, 2017, 65(43): 9559-9567.
2. Zhang X K, Li S Y, Zhao X,et al. HPLC-MS/MS-based targeted metabolomic method for profiling of malvidin derivatives in dry red wines. Food Research International, 2020, 134: 109226.
3. Gutierrez E, García Villaraco A, et al. Transcriptomics, targeted metabolomics and gene expression of blackberry leaves and fruits indicate flavonoid metabolic flux from leaf to red fruit. Frontiers in plant science, 2017, 8: 472.