Integrative Metabolome and Microbiome Analysis
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Microbiome sequencing, mainly including amplicon sequencing, full-length amplicon sequencing and metagenome sequencing, provides information on bacterial composition, gene abundance and functionality. Metabolomics studies metabolite changes in organisms. The complexity of the bioscience research process is such that a single histology alone and one-sided cannot explain the biology clearly, and multi-omics becomes particularly important. Combining microbiome and metabolomics to jointly explain scientific questions at the species, gene, and metabolite levels provides a better understanding of disease processes and the metabolic pathways of substances in the body, and also helps to identify biomarkers of disease for further application in clinical aids to diagnosis.
Creative Proteomics has established a combined microbiome and metabolome analysis platform that can be applied to analyze disease markers, study disease mechanisms and prevention, study drug metabolism, and phenotype-related analysis.
Analysis Projects for
Integrative Metabolome and Microbiome| Microbiome | 16S rDNA fragment sequencing and 16S rDNA full-length sequencing |
| Metagenome sequencing |
| Metabolome | Untargeted metabolomics and targeted metabolomics |
Technology Roadmap for
Integrative Metabolome and Microbiome
Sample Submission
| Sample size | Instrument |
| Microbiome analysis samples | Total DNA: metagenomic analysis ≥ 2μg; 16S sequencing ≥ 500ng
Feces, intestinal contents, tissues: ≥ 1g/sample
Ruminal fluid, fermentation fluid, tissue fluid (with obvious sedimentation by centrifugation): ≥ 3 mL (1 g of sedimentation).
For other sample types, please consult the relevant staff. | PE150, PE250 |
| Metabolome analysis samples | Serum and plasma: 200 ul/sample. If hemolysis occurs, resampling is recommended.
Urine: 500 ul/sample
Tissue: 200 mg/sample
Cells: ≥ 1×107/sample
Cerebrospinal fluid: 200 ul/sample
Stool and intestinal contents: 500 mg/sample
For other sample types, please consult the relevant staff. | Mass spectrometric detection |
| Note | Stored at -80℃ after liquid nitrogen flash-freezing, sent on dry ice. Avoid repeated freezing and thawing. |
Advantages for
Integrative Metabolome and Microbiome| Advanced platforms | Sample extraction and quality control, experiments, basic analysis and advanced analysis are integrated, making the service more professional and efficient.
Provide basic and advanced analysis of microbiome and metabolome. |
| One-stop service | Sample extraction and quality control, experiments, basic analysis and advanced analysis are integrated, making the service more professional and efficient. |
| Comprehensive analysis | Provide basic and advanced analysis of microbiome and metabolome. |
If you want to know more, please contact us. Looking forward to cooperating with you.
For Research Use Only. Not for use in diagnostic procedures.
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