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Flavonoids Analysis Service

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What are Flavonoids?

Flavonoids are a class of plant secondary metabolites with the structure of 2-phenyl-chromones, which are widely found in the plant kingdom. They are usually combined with sugars in plants to form glycosides in the form of ligands, and to a lesser extent in the form of free sapogenins. Flavonoids not only play an important role in the growth and development of plants, but also have pharmacological activities such as antibacterial, antiviral, antitumor, antioxidant free radical, anti-inflammatory and analgesic, anticancer, anti-aging, anti-cardiovascular disease, anti-radiation, hepatoprotective and treatment of osteoporosis.

To promote flavonoid research in the synthesis of new biological drugs and medical applications, Creative Proteomics has established a flavonoid analysis platform that enables efficient and accurate identification and detection of flavonoid content changes using the UHPLC-Q-Exactive MS analytical instrument. We assist in exploring novel flavonoids, clarifying their anti-disease mechanisms, and facilitating research related to enhancing their bioactivity and utilization through structural modifications. Optimized extraction and purification techniques can be used to clarify the conformational relationships by various methods and facilitate the optimization of flavonoid production processes.

Types of Flavonoids We Can Detect Include But Are Not Limited To

Types of FlavonoidsSpecific Substances
FlavonesApigenin, Lignanin, Luteolin, Baicalin, Baicalein, Tangeritin, Polymethoxylated Flavone
FlavonolPrunetin, Isorhamnetin, Hammaritin, Kaempferol, Quercetin, Myricetin, Fisetin
Flavanone (Dihydroflavone)Hypericin, Hesperidin, Eriodictyol, Naringenin
Flavanonol (Dihydroflavonol)Dihydrokaempferol, Taxifolin, Sangenone C/D
IsoflavoneGenistein, Puerarin, Soy isoflavones, Glycitein, Daidzein
Isoflavanone (Dihydroisoflavone)
ChalconeCrocin, Sophoradin
AnthocyanidinCyanidin, Delphindin, Pelargonidin, Malvin, Peonidin, Petunidin
FlavanolsCatechins, Epicatechin, Gallocatechin
BiflavoneGinkgetin, Bilobetin

Flavonoid Profiles Analysis Platform in Creative Proteomics

UHPLC-Q-Exactive MS combines the high separation efficiency of UHPLC with the accurate mass determination and high-resolution capabilities of a Quadrupole-Orbitrap Mass Spectrometer. This tandem technique allows for the sensitive and specific detection, identification, and quantification of flavonoids in complex matrices. The UHPLC-Q-Exactive MS analysis features:

1. High Separation Efficiency with UHPLC

UHPLC employs columns with small particle sizes and high-pressure systems, enabling rapid and efficient separation of complex mixtures. The increased resolution and sensitivity of UHPLC facilitate the separation of closely related flavonoids and reduce interferences from matrix components. This high separation efficiency enhances the reliability and accuracy of flavonoid identification and quantification.

2. Accurate Mass Determination with Quadrupole-Orbitrap MS

The Quadrupole-Orbitrap Mass Spectrometer in the UHPLC-Q-Exactive MS system provides accurate mass determination and high-resolution data. By acquiring full scan spectra and targeted tandem MS experiments, the instrument can precisely measure the molecular weight and fragmentation patterns of flavonoids. This information aids in the identification and structural elucidation of flavonoid compounds, including their glycosylation patterns and positional isomers.

3. Metabolite Profiling and Dereplication

Enables comprehensive metabolite profiling of flavonoids in plant extracts or biological samples. By comparing the obtained mass spectra with spectral libraries or databases, the technique allows for rapid identification and dereplication of known flavonoid compounds. Moreover, it facilitates the discovery of novel flavonoid metabolites or derivatives, expanding our understanding of their chemical diversity.

4. Quantitative Analysis and Profiling

Quantitative analysis of flavonoids is essential for evaluating their content in plant extracts or finished products. UHPLC-Q-Exactive MS enables accurate and precise quantification of flavonoid compounds using internal standards and calibration curves. This quantitative profiling provides valuable insights into the variations of flavonoid content among different plant sources, growth conditions, or processing techniques.

What We Can Provide:

  • Method Development and Optimization
  • Structure Elucidation of Flavonoids: The combination of UHPLC-Q-Exactive MS and Creative Proteomics' expertise allows for the structure elucidation of flavonoids. We utilize precise mass measurements, fragmentation analysis, and spectral interpretation to determine chemical structures and identify key structural features.
  • Targeted and Untargeted Analysis: We provide targeted analysis to quantify specific flavonoid compounds of interest. In addition, untargeted analysis allows the discovery and analysis of a broad range of flavonoid compounds, both known and unknown. This comprehensive approach reveals the complete flavonoid composition.
  • Data Analysis and Interpretation: We are able to provide our clients with detailed reports and results of data analysis.

Workflow of Flavonoids Analysis


Sample Requirements

  • Serum / plasma ≥ 100 ul/sample
  • Animal tissue: ≥ 50 mg/sample
  • Urine: ≥ 100 ul/sample
  • Feces: ≥ 50 mg/sample
  • Saliva: ≥ 100 ul /sample
  • Plant samples: ≥ 200 mg
  • Cells: 1x107/sample

We can also provide other sample types for analysis. Please contact our technicians for specific sample types and content requirements.

Report Delivery

  • Experimental procedure
  • Parameters of liquid chromatography and mass spectrometry
  • MS raw data files and MS data quality checks
  • Flavonoid quantification data
  • Custom analysis report

Creative Proteomics offers several approaches to provide flavonoids analysis service and deliver precise and detailed data and analysis report. If you want to detect other compounds, you can tell us through the inquiry form, and our technicians will communicate with you.

For Research Use Only. Not for use in diagnostic procedures.


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