Energy metabolism means all the reactions involved in generating adenosine triphosphate (ATP) from nutrients including both aerobic respiration (oxygen present) and anaerobic respiration (fermentation) (Figure 1). Their production begins with catabolism of large molecular substances. The fragments generated by this process are converted to basic intermediates (like acetyl-CoA), which are further oxidised in citric acid cycle and the resulting coenzymes (NADH + H+ a FADH2) are used in electron transport chain to produce ATP. So far, the energy metabolism of mammalians includes three different forms: oxidative phosphorylation, nitrogen metabolism and sulfur metabolism. The energy metabolism plays an important role in cells' fundamental functions like proliferation and respiration. Its aberrant is closely associated with various diseases like inborn errors of metabolism and Lysosomal Storage Disease. In Creative Proteomics, we provide reliable, rapid and cost-effective energy metabolism analysis service to speed up diseases diagnosis and gain new insights in disease mechanism or treatment.
Figure 1. Simplified example of energy metabolism of glucose in the cell.
The Main Applications of Energy Metabolism Service
- Identifying novel biomarkers of diseases
- Improving diagnose diseases
- Accelerating development of drugs
- Gaining new mechanism of metabolic diseases
Advantages of Our Energy Metabolism Service
Ample experience in a variety of animal sample preparation
Advanced core facilities
Professional experiment design and data analysis
Short experimental time
High accuracy, specificity, and sensitivity
Creative Proteomics provides energy metabolism service in a reliable and effective manner, based on our cutting-edge high-performance liquid chromatography- mass spectrometry (HPLC-MS) platforms. The experimental procedures contain four major stages: sample collection, metabolites extraction, HPLC-MS data analysis and bioinformatics analysis. Our service will be tailored to specific samples and needs for optimal results.
Figure 2. The overall workflow of energy metabolism service.
The Potential Detectable Energy Metabolism Related Metabolites at Creative Proteomics
We can analyze a wide range of biological materials including but not limited to cells and solid tissues from humans and animals, such as mice, rats, rabbits. If you need transport your samples to us, please follow the below requirements for different kinds of sample:
- Blood/plasma: 500ul/sample
- Urine: 1ml/sample
- Tissue: 200mg/sample
- Cells: 1x107/sample
- Feces: 500mg/sample
Shipment condition: dry ice
- Experimental protocols
- Instrumental factors of HPLC
- The raw data files of HPLC and the summary of HPLC data quality
- Bioinformatics analysis report
Based on advanced HPLC platforms for the determination of energy metabolism, professional bioinformatic analysis software and experienced technicians and scientists, Creative Proteomics provides customer-tailored energy metabolism analysis service with rapid experimental procedures and easy to read report, to accelerate your scientific research.
- Fernie Alisdair R, Carrari Fernando, Sweetlove Lee J. Respiratory metabolism: glycolysis, the TCA cycle and mitochondrial electron transport. Curr Opin Plant Biol. 2004.7:254-261.
- El-Hattab Ayman W. Inborn errors of metabolism. Clin Perinatol. 2015.42:413-439.
- Platt Frances M, d'Azzo Alessandra, Davidson Beverly L et al. Lysosomal storage diseases. Nat Rev Dis Primers. 2018.4:27.
For Research Use Only. Not for use in diagnostic procedures.