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Glyphosate and Metabolites Analysis

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What is Glyphosate and its Metabolites?

Glyphosate is a widely recognized herbicide, originally introduced in the 1970s, that operates as a broad-spectrum herbicide, demonstrating its effectiveness in controlling a wide range of plant species. The herbicide's mechanism of action is achieved by inhibiting 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS), an enzyme intricately involved in the synthesis of aromatic amino acids in plants. This herbicide function leads to the inability of the plant to grow and ultimately to its eventual death.

In addition, glyphosate is used as a desiccant for several crops, including wheat and soybeans. As a result of the metabolism of this herbicide in plants and animals, various glyphosate compounds are produced, including aminomethylphosphonic acid (AMPA) and glyphosate-phthalic acid (GOA). These compounds can persist in the environment and can be located in several environmental matrices, such as soil, water, and food. AMPA is the most common glyphosate metabolite found in soil and water matrices, while GOA is often found in foods, such as soybeans.

Why is Glyphosate and its Metabolites Analysis Important?

The apprehension surrounding the plausible health and environmental ramifications of glyphosate and its metabolites is escalating. Glyphosate and its metabolites have been detected in a plethora of environmental specimens globally, comprising of soil, water, air, and food. In 2015, the International Agency for Research on Cancer (IARC), based on the minuscule proof from animal studies and humans, categorized glyphosate as "possibly carcinogenic to humans." The apprehensions don't end here; there are serious concerns about the aftermath of glyphosate and its metabolites on non-target organisms such as honeybees and other pollinators.

The comprehensive analysis of glyphosate and its metabolites services can be instrumental in assessing the levels of glyphosate and its metabolites in sundry environmental matrices, including soil, water, and food. Such services can help to estimate the potential risks of glyphosate and its metabolites to human health and the environment, as well as appraise the effectiveness of glyphosate-based herbicides and trace potential sources of contamination. Additionally, this analysis provides information on the persistence and fate of glyphosate and its metabolites in the environment.

List of Glyphosate and Metabolites, including but not limited to:

Glyphosate and Metabolites
GlyphosateGlycine3,6-Dichloro-2-hydroxybenzoic acid (D2HBA)
Aminomethylphosphonic acid (AMPA)Sarcosine3,6-Dichlorosalicylic acid (3,6-DCSA)
N-acetylglyphosate (NAG)3-(Methylthio)propionic acid (MMPA)4-Chloro-3-methylphenol (CMP)
N-acetyl-AMPA (NA-AMPA)2-Oxopropylphosphonic acid (OPPA)2-Hydroxy-3-[(phosphonooxy) methyl]benzoic acid (HOPDA)

Technology Platform for Creative Proteomics's Glyphosate and Metabolites Analysis

Creative Proteomics provides analytical services for glyphosate and its metabolites based on LC-MS technology, including sample preparation, method development and analysis. A wide range of environmental matrices can be analyzed, including soil, water and food. Our team of experienced scientists can provide reliable and accurate results for a wide range of environmental samples.

Our LC-MS platform is equipped with high-resolution mass spectrometry (HRMS) and triple quadrupole mass spectrometry (QQQMS) systems to detect and quantify glyphosate and its metabolites at trace levels. the LC-MS platform is also coupled with a variety of sample preparation techniques, including solid phase extraction (SPE) and liquid-liquid extraction (LLE), to extract from a variety of matrices, including soil, water and food Glyphosate and its metabolites.

Workflow for Glyphosate and Metabolites Analysis

Workflow for Glyphosate and Metabolites Analysis

Sample Requirements

  • Soil samples: a minimum of 500 grams of soil, which should be collected at a depth of 0-15 cm.
  • Water samples: a minimum of 500 mL of water.
  • Food samples: a minimum of 100 grams of the sample, which should be homogenized prior to analysis.

If you would like to analyze other metabolites or learn more, please contact us. We look forward to working with you.

For Research Use Only. Not for use in diagnostic procedures.


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