Metabolomics Creative Proteomics

Free Fatty Acids Analysis Service

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Free fatty acids (FFA) are both the product of fat hydrolysis and the substrate of fat synthesis. The concentration of FFA is related to lipid metabolism, sugar metabolism, endocrine function, and can also reflect changes in food quality during food storage.

FFA only accounts for 10% of total fatty acids in biological samples. With its low content and wide dynamic fluctuation range, it is a challenge to simultaneously detect and quantify as much FFAs as possible in biological samples at one time. We use LC-MS/MS to qualitatively and quantitatively detect the full spectrum of FFAs in biological samples such as urine, whole blood/serum/plasma. The detection of FFA levels to reflect the body's lipid metabolism, sugar metabolism and endocrine function status is of great significance for exploring the pathogenesis and prevention of diseases.

FFAs can have an important effect on food flavor. The content of FFAs in the oil is an important indicator of the quality of the oil. Testing the FFA content of food products can help evaluate product quality.

Free Fatty Acids Analysis Service

According to different analysis needs, Creative Proteomics provides a series of fatty acid analysis services based on GC-MS and LC-MS. We can customize exclusive solutions for you according to your samples.

Service Workflow

The workflow of free fatty acids analysisThe workflow of free fatty acids analysis

Mode: SRM / MRM
Analysis content:

  • Standard curve creation
  • Raw data preprocessing
  • Absolute quantification of fatty acids

List of Detectable Free Fatty Acids at Creative Proteomics

Sample Requirements

  • Serum / plasma ≥ 100 ul/sample
  • Animal tissue: ≥ 50 mg/sample
  • Saliva: ≥ 100 mg/sample
  • Bile: ≥ 10 ul/sample
  • Cell: ≥ 1×107
  • Plant samples: ≥ 200 mg
  • Yeasts, microorganisms, etc.: dry weight ≥ 200 mg
  • Fats, oils, flours, nuts, animal foods, mixes: ≥ 100g

Quick-freeze in liquid nitrogen and store at -80°C, and send it on dry ice.
Please contact us for other sample types and dosages.


  • Experimental procedure
  • Parameters of liquid chromatography / gas chromatography and MS
  • MS raw data files and MS data quality checks
  • Free fatty acids quantification data
  • Custom analysis report

Turn Around Time: 1-3 weeks

Creative Proteomics offers several approaches to provide free fatty acids analysis service and deliver precise and detailed data and analysis report. If you want to detect other metabolites but have not found them, you can tell us through the inquiry form, and our technicians will communicate with you.

For Research Use Only. Not for use in diagnostic procedures.


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