Vitamins Quantitative Analysis Service

Vitamins are a class of trace organic substances that humans and animals must obtain from food to maintain normal physiological functions and play an important role in human growth, metabolism and development. Depending on their chemical structure and water solubility, vitamins are divided into two categories: water-soluble vitamins and fat-soluble vitamins.

Accurate determination of vitamin content plays an important role in controlling food nutrition and safety. Determination of vitamins in the livestock industry also helps to develop better nutritional feeds and to explore disease, metabolism and other related mechanisms. In addition, vitamin analysis is also needed to study the mechanisms of various human diseases and to enable drug development.

Some vitamin samples may have complex matrices, including fats, emulsifiers, proteins, sterols and other fat-soluble substances, and some vitamins are unstable and require special handling. Creative Proteomics has optimized pre-treatments and assays for different types of vitamins. We have developed highly sensitive, selective and accurate LC/MS/MS analytical methods for the identification and accurate quantification of vitamins in complex matrices.

Service Workflow

Instrument platform: AB Sciex QTRAP® 6500 LC-MS/MS

List of Partial Detectable Vitamins at Creative Proteomics

Application of Vitamins Quantitative Analysis

• Applications in multiple fields such as agronomy, medical physiology, and nutrition
• Research on disease control mechanism
• Analysis of plant vitamin metabolism and regulation
• Research on plant vitamin biosynthesis pathways
• Analysis of bioactive functions (antioxidant, antitumor)

Sample Requirements

Acceptable sample types: animal and plant tissues, cells, blood, etc.

• Blood/plasma: ≥ 500ul/sample
• Urine: ≥ 1ml/sample
• Tissue: ≥ 200mg/sample
• Cells: ≥ 1x10⁷/sample
• Feces: ≥ 500mg/sample

It is recommended to repeat more than six. Large pieces need to be cut into 1cm³ pieces.

Shipment condition: dry ice.

Report Delivery

• Experimental procedure
• Parameters of liquid chromatography and mass spectrometry
• Raw data and quality control of liquid chromatography and mass spectrometry
• Metabolite quantification data
• Custom analysis report

Creative Proteomics has leading-edge technology and best-in-class R&D and manufacturing facilities to help you plan, conduct and report metabolomics studies, making your upstream and downstream experiments seamless. If you have any questions or specific requests, please feel free to contact us.