Metabolomics Creative Proteomics

Creative Proteomics Metabolomics

Nicotinate and Nicotinamide Analysis Service

Nicotinate and Nicotinamide Analysis Service

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Creative Proteomics is a pioneer in metabolic analysis service and provides reliable, professional and cost-effective nicotinate and nicotinamide metabolism analysis service in animal. We leverage our rich experience, professional expertise, and advanced technologies to speed up your scientific purposes of nicotinate and nicotinamide metabolism analysis in animal.

Overview

Nicotinate and nicotinamide are precursors of the nicotinamide-adenine dinucleotide (NAD+) and its phosphorylated form, NADP+,which are essential for redox reactions and carry electrons from one reaction to another. NAD+ synthesis happens either in vivo from amino acids or a salvage pathway from nicotinamide. The savage pathway is typically found in mammals. The specificity of the in vivo pathway varies among organisms, but most start by forming quinolinic acid (QA) from tryptophan (Trp) in animals. The salvage pathway involves recycling nicotinamide and nicotinamide-containing molecules like nicotinamide riboside. NAD+ and NADP+ act as cofactors in multiple reduction-oxidation (redox) reactions related to energy production, glycolysis, the tricarboxylic acid cycle, oxidative phosphorylation and fatty acid oxidation. The alterations of nicotinate and nicotinamide metabolism is associated with multiple human diseases like cancer and cardiovascular diseases. Scientists at Creative Proteomics take advantage of highly quantitative methods with high-performance liquid chromatography (HPLC) for the determination of nicotinate and nicotinamide metabolism related metabolites levels in various samples. Furthermore, we provide reliable, rapid and cost-effective nicotinate and nicotinamide metabolism related service to speed up your research aims ranging from diseases diagnosis to gaining new insights in disease mechanism or treatment.

The Main Applications of Nicotinate and Nicotinamide Analysis Service

  • Identifying novel biomarkers of diseases
  • Improving diagnose diseases
  • Accelerating development of novel drugs
  • Gaining new mechanism of diseases

Advantages of Our Nicotinate and Nicotinamide Analysis Service

  • Constantly optimized protocol and analytical software
  • Professional experiment design
  • Quick turnaround time
  • High accuracy, specificity, and sensitivity

Service Workflow

Creative Proteomics provides nicotinate and nicotinamide metabolism analysis service in a reliable and effective manner, based on our cutting-edge HPLC platforms. The experimental procedures contain four main procedures: sample collection, metabolites extraction, HPLC data analysis and bioinformatics analysis (Figure 1). Our service will be tailored to specific samples and needs for optimal results.

The overall workflow of nicotinate and nicotinamide metabolismFigure 1. The overall workflow of nicotinate and nicotinamide metabolism

List of Partial Detectable Nicotinate and Nicotinamide Metabolism Related Metabolites at Creative Proteomics

NicotinamideNicotinamide-adenine dinucleotideNicotinamide D-ribonucleotide
NicotinateNicotinamide-adenine dinucleotide phosphateTrigonelline
NicotineHydroxynicotinateNicotinurate

Sample Requirements

We can analyze a wide range of biological materials including but not limited to cells and solid tissues from animals. If you need transport your samples to us, please follow the following requirements for different types of sample:

  • Blood/plasma: 500ul/sample
  • Urine: 1ml/sample
  • Tissue: 200mg/sample
  • Cells: 1x107/sample
  • Feces: 500mg/sample
    Shipment condition: dry ice

Report Delivery

  • Experimental protocols
  • Instrumental factors of HPLC
  • The raw data files of HPLC and the summary of HPLC data quality
  • Bioinformatics analysis report

Based on advanced HPLC platforms for the determination of nicotinate and nicotinamide metabolism related metabolites, professional bioinformatic analysis software and experienced technicians and scientists, Creative Proteomics provides customer-tailored nicotinate and nicotinamide metabolism analysis service with rapid experimental procedures and easy to read report, to accelerate your scientific research.

References

  1. Chiarugi Alberto, Dölle Christian, Felici Roberta et al. The NAD metabolome--a key determinant of cancer cell biology. Nat Rev Cancer. 2012 12:741-752.
  2. Yaku Keisuke, Okabe Keisuke, Hikosaka Keisuke et al. NAD Metabolism in Cancer Therapeutics. Front Oncol. 2018.12;8:622.
For Research Use Only. Not for use in diagnostic procedures.

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