Metabolomics Creative Proteomics

Creative Proteomics Metabolomics

Vitamin B6 Analysis Service

Vitamin B6 Analysis Service

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Creative Proteomics is a pioneer in metabolic analysis service and provides reliable, professional and cost-effective vitamin B6 metabolism analysis service in animal. We leverage our rich experience, professional expertise, and advanced technologies to speed up your scientific purposes of vitamin B6 metabolism analysis in animal.

Overview

Vitamin B6 is one of the B vitamins,which is water-soluble. Vitamin B6 occurs in various forms, one of which is an active form is pyridoxal phosphate (PLP). PLP functions as cofactor in a variety of reactions including from amino acid metabolism to lipid metabolism. PLP together with pyridoxine-5'-phosphate (PNP) and pyridoxamine-5'-phosphate (PMP) are generated intracellularly by pyridoxal kinase (PDXK), which catalyses the conversion of three non-phosphorylated vitamin B6 precursors, respectively. The dephosphorylation of PNP, PMP and PLP is mediated by the PDXK-antagonistic enzyme pyridoxal phosphatase (PDXP). In addition, PN, PM and PL as well as their phosphorylated forms can be converted into each other due to the activity of PMP oxidase. In animals, PLP catabolism mainly proceeds via the aldehyde oxidase 1-mediated generation of 4-pyridoxic acid, which is excreted in urine. It would not be surprising that aberrant vitamin B6 metabolism has been connected with various human diseases like cancer and diabetes. Scientists at Creative Proteomics take advantage of highly quantitative methods with high-performance liquid chromatography (HPLC) for the determination of vitamin B6 metabolism related metabolites levels in various samples. Furthermore, we provide reliable, rapid and cost-effective vitamin B6 metabolism related service to speed up your research aims ranging from diseases diagnosis to gaining new insights in disease mechanism or treatment.

The Main Applications of Vitamin B6 Analysis Service

  • Identifying novel biomarkers of diseases
  • Improving diagnose diseases
  • Accelerating development of novel drugs
  • Gaining new mechanism of diseases

Advantages of Our Vitamin B6 Analysis Service

  • Constantly optimized protocol and analytical software
  • Professional experiment design
  • Quick turnaround time
  • High accuracy, specificity, and sensitivity

Service Workflow

Creative Proteomics provides vitamin B6 metabolism analysis service in a reliable and effective manner, based on our cutting-edge HPLC platforms. The experimental procedures contain four main procedures: sample collection, metabolites extraction, HPLC data analysis and bioinformatics analysis (Figure 1). Our service will be tailored to specific samples and needs for optimal results.

The overall workflow of vitamin B6 metabolismFigure 1. The overall workflow of vitamin B6 metabolism

List of Partial Detectable Vitamin B6 Metabolism Related Metabolites at Creative Proteomics

Pyridoxal-5'-phosphatePyridoxine
Pyridoxine-5'-phosphatePyridoxamine
Pyridoxamine-5'-phosphatePyridoxal

Sample Requirements

We can analyze a wide range of biological materials including but not limited to cells and solid tissues from animals. If you need transport your samples to us, please follow the following requirements for different types of sample:

  • Blood/plasma: 500ul/sample
  • Urine: 1ml/sample
  • Tissue: 200mg/sample
  • Cells: 1x107/sample
  • Feces: 500mg/sample
    Shipment condition: dry ice

Report Delivery

  • Experimental protocols
  • Instrumental factors of HPLC
  • The raw data files of HPLC and the summary of HPLC data quality
  • Bioinformatics analysis report

Based on advanced HPLC platforms for the determination of vitamin B6 metabolism related metabolites, professional bioinformatic analysis software and experienced technicians and scientists, Creative Proteomics provides customer-tailored vitamin B6 metabolism analysis service with rapid experimental procedures and easy to read report, to accelerate your scientific research.

References

  1. Galluzzi L, Vacchelli E, Michels J. et al. Effects of vitamin B6 metabolism on oncogenesis, tumor progression and therapeutic responses. Oncogene. 2013. 32:4995-5004.
  2. Mocellin Simone, Briarava Marta, Pilati Pierluigi. Vitamin B6 and Cancer Risk: A Field Synopsis and Meta-Analysis. J Natl Cancer Inst. 2017. 109:1-9.
For Research Use Only. Not for use in diagnostic procedures.

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