Metabolomics Creative Proteomics

Creative Proteomics Metabolomics

Porphyrin and Chlorophyll Metabolism Service

Porphyrin and Chlorophyll Metabolism Service

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Porphyrin is a class of macrocyclic aromatic compound comprised of four pyrrole rings connected by methine bridges. Porphyrin is ubiquitous in nature and functions as a heme cofactor of hemoglobin, cytochromes and other redox active enzymes. In animals, the committed step for porphyrin biosynthesis is the formation of 5-aminolevulinic acid via the reaction of the amino acid glycine with succinyl-CoA from the citric acid cycle. As the precursors of heme, the overproduction of porphyrins is closely associated with the pathology of porphyrias. In contrast, chlorophyll is vital for photosynthesis, allowing plants to absorb energy from light. The majority of anima cells do not contain chlorophyll. Pheophytins and pheophorbides are the main products detected in the liver following chlorophyll intake in animals, indicating that chlorophyll could be beneficial for animal's health. In addition, metallo-chlorophyll derivatives have been reported to be involved in modulation of oxidative stress, xenobiotic metabolism and gene expression. Therefore, the insight into porphyrin and chlorophyll metabolism contribute to know the pathology of certain human diseases. Scientists at Creative Proteomics take advantage of highly quantitative methods with high-performance liquid chromatography (HPLC) for the determination of porphyrin and chlorophyll metabolism related metabolites levels in various samples. Furthermore, we provide reliable, rapid and cost-effective porphyrin and chlorophyll metabolism related service to speed up your research aims ranging from diseases diagnosis to gaining new insights in disease mechanism or treatment.

The Main Applications of Porphyrin And Chlorophyll Metabolism Service

  • Identifying novel biomarkers of diseases
  • Improving diagnose diseases
  • Accelerating development of novel drugs
  • Gaining new mechanism of diseases

Advantages of Our Porphyrin And Chlorophyll Metabolism Service

  • Constantly optimized protocol and analytical software
  • Professional experiment design
  • Quick turnaround time
  • High accuracy, specificity, and sensitivity.

Service Workflow

Creative Proteomics provides porphyrin and chlorophyll metabolism analysis service in a reliable and effective manner, based on our cutting-edge HPLC-MS platforms. The experimental procedures contain four main procedures: sample collection, metabolites extraction, HPLC-MS data analysis and bioinformatics analysis (Figure 1). Our service will be tailored to specific samples and needs for optimal results.

The overall workflow of porphyrin and chlorophyll metabolismFigure 1. The overall workflow of porphyrin and chlorophyll metabolism

List of Partial Detectable Porphyrin And Chlorophyll Metabolism Related Metabolites at Creative Proteomics

Glycine5-AminolevulinatePorphobilinogenProtoporphyrin
HydroxymethylbilaneUroporphyrinogen IIICoproporphyrinogen IIIProtoheme

Sample Requirements

We can analyze a wide range of biological materials including but not limited to cells and solid tissues from animals. If you need transport your samples to us, please follow the following requirements for different types of sample:

  • Blood/plasma: 500ul/sample
  • Urine: 1ml/sample
  • Tissue: 200mg/sample
  • Cells: 1x107/sample
  • Feces: 500mg/sample

Shipment condition: dry ice

Report Delivery

  • Experimental protocols
  • Instrumental factors of HPLC
  • The raw data files of HPLC and the summary of HPLC data quality
  • Bioinformatics analysis report

Based on advanced HPLC platforms for the determination of porphyrin and chlorophyll metabolism related metabolites, professional bioinformatic analysis software and experienced technicians and scientists, Creative Proteomics provides customer-tailored porphyrin and chlorophyll metabolism analysis service with rapid experimental procedures and easy to read report, to accelerate your scientific research.

References

  1. E. Pérez-Urria, A. Avalos. Approach to a comparative study of the metabolism of porphyrins and chlorophylls. J Nat Sci. 2015.3:1-16.
  2. Rumpho Mary E, Pelletreau Karen N, Moustafa Ahmed et al. The making of a photosynthetic animal. J Exp Biol. 2011.214:303-311.
For Research Use Only. Not for use in diagnostic procedures.

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