Microbial Metabolomics Service

The microbial genome is small, and the data obtained by genomics, transcriptomics, and proteomics are relatively small compared to eukaryotes, and metabolome research will not be affected by this. There are probably at least 20,000 kinds of microbial metabolites that have been elucidated. With the aid of microbial cell metabolite full-component analysis, it can reflect the cell's response to environmental and genetic changes, and promote a comprehensive understanding of the physiological functions of microorganisms.

There is a close relationship between the microbial flora and the host. For example, changes in the composition of the intestinal flora and its metabolites may be related to various factors such as disease, obesity, and diet. Analyzing the microbial metabolome can help to find the microorganisms and metabolites that play a key role in the physiological and disease processes, and reveal the mechanisms of physiological processes and disease occurrence and development.

Microbial metabolomics has been widely used in different research fields, such as the identification and mutation breeding of microorganisms, functional gene research, metabolic engineering and fermentation engineering.

Based on high-resolution mass spectrometry technology, Creative Proteomics has established a metabolomics research platform for microbial primary metabolites and secondary metabolites, help you explore the relationship between biological problems and metabolites and microorganisms, find highly related metabolites and microorganisms, and then explore the internal network of metabolites, microorganisms and biological background. We will customize different solutions according to your samples to accurately identify and quantitatively analyze microbial metabolites.

Metabolomics Solutions Analysis Strategies

Untargeted Metabolomics Service

Perform a comprehensive, non-focused qualitative and quantitative analysis of the sample. Find out different metabolites through comparison, and combine with biological information analysis to find out enrichment differential pathways.

Targeted Metabolomics Service

Determination of the absolute concentration of the target metabolite of the sample.

Metabolomics Solutions Research Project

Research on disease markers, disease mechanism, and drug metabolism

Screening and identification of strains, analysis of metabolic pathways, fermentation engineering and probiotic effects

Food safety evaluation, nutrition evaluation, etc.

Research on gut microbiome and metabolomics in host physiology, disease pathology, drug pharmacology, etc.

Drug resistant bacteria detection

Research on biofilm formation, drug resistance mechanism and anti-biofilm agents

Vibrio Cholerae Metabolomics

Staphylococcus Aureus Metabolomics

Parasite Metabolomics

Instrumentation

Creative Proteomics employs state-of-the-art LC-MS/MS and GC-MS/MS systems for microbial metabolomics studies.

Our primary instruments include:

• AN Sciex Qtrap 6500 Plus
• AB 5600 Triple TOF
• Agilent 7890-5977
• Thermo TSQ^TM 9000

Workflow for quantitative microbial metabolome analysis (van et al., 2007)

Metabolomics Data Analysis

Reference

1. van der Werf, M. J., Overkamp, K. M., et al. (2007). Microbial metabolomics: toward a platform with full metabolome coverage. Analytical biochemistry, 370(1), 17-25.