Overview
Flavanones, also called bioflavonoids, are a class of compounds with 2-phenyldihydrochromone as the core. Flavonoids are derivatives of chromanone and chroman, and flavanones have a structure of C6-C3-C6.
Flavanones are widely found in nature and are one of the active ingredients of many folk medicinal plants such as sweet root and red bluebell. Flavanone derivatives are mostly extracted from natural plants, and the substituents are mostly hydroxyl, methoxy, benzyloxy, isopentenyl, geranyl, etc. Due to their significant pharmacological and physiological activities as well as their unique plastic structure, their research has been paid more and more attention. Many flavanone compounds have many activities such as bactericidal, anti-inflammatory, anti-tumor, anti-HIV virus, anti-mutagenic, antioxidant, etc. They are a class of compounds with high research value.
Flavanone compounds are important active ingredients of natural medicines. For a long time, people have hoped to seek chemical models from natural products to research and develop new drugs, and to make reasonable structural modifications to the immune substances produced by plants, so as to screen out new compounds with high activity. There are a total of 10 positions that can be substituted on the flavanone matrix, which has great potential for structural modification. And flavanones are intermediates in the synthesis of a variety of flavonoids.
The biological activity of flavanone compounds has received extensive attention. Creative Proteomics has established an efficient, fast, accurate and scientific analysis platform to realize the qualitative and quantitative analysis of flavanones. UPLC-MS/MS is a sensitive analytical technique to characterize the chemical structure of various flavanones for detection, identification and quantification at low abundance.
Applications of Flavanones Analysis
- Reveal mechanisms of plant development
- Uncover plant's responses to biotic and abiotic stresses
- Safety assessment of crops
- Crop improvement
Advantages of Our Flavanones Analysis Service
Rich experience in plant sample handling and analysis of various flavanones
Enable analysis of flavanones present in a variety of plant species at cell/tissue level.
Accurate quantification of flavanones with UPLC-MS/MS
Flexible statistical analysis and bioinformatics analysis
Service Workflow
Since different tissues have different matrix properties, Creative Proteomics has developed multiple novel sample preparation techniques. Our services allow the profiling of multiple flavanones, including catechin.
Analysis patterns: multi-response monitoring (MRM) mode under electrospray positive ion scanning
Linear: R>0.99
Time: dozens of minutes, slightly different according to different ingredients
Analysis content:
- Standard curve creation
- Raw data preprocessing
- Absolute quantification of flavanones
- Differential coumarins screening
- Optimal analyses
List of Detectable Flavanones at Creative Proteomics
| Catechin | Naringenin | Farrerol |
| Oxazolidine | Neohesperidin | Proanthocyanidin |
| Flavan-3-ol | Flavan-4-ol | Flavane-3,4-diol |
Sample Requirements
- Plant tissue > 1 g / sample
- Animal tissue 200 mg / sample
- Animal body fluid samples > 200 µl
- In addition, drug, food and juice samples can also be analyzed.
The samples should be frozen in liquid nitrogen immediately after collection, and then transferred to -80°C for storage. In order to ensure the accuracy of the samples and reduce systematic errors during sampling, it is necessary to select more than 3 materials with the same condition for each sample. Please contact our technicians for specific testing requirements.
Deliverables
- Experimental procedure
- Parameters of UPLC-MS/MS
- Purity analysis report
- UPLC-MS/MS raw data files and UPLC-MS/MS data quality checks
- Metabolites quantification data
- Custom analysis report
UPLC-MS/MS-based profiling of flavanones enables quantitative analyses of flavanones in a faster, convenient, and sensitive manner. With decades of experience in UPLC-MS/MS services, Creative Proteomics has a proven track record supporting diverse flavanones detection and quantification. We can meet your specific project requirements, from sampling to bioinformatics.
For Research Use Only. Not for use in diagnostic procedures.
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