Metabolomics Creative Proteomics

Creative Proteomics Metabolomics

Flavanones Analysis

Flavanones Analysis

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Creative Proteomics provides one-stop ultrahigh performance liquid chromatography coupled with triple-quadrupole tandem mass spectrometry (UPLC-MS/MS) services covering a wide variety of plant targeted metabolomics. Our flavanones analysis services use UPLC-MS/MS technologies to deliver high resolution and reliable identification and quantification of flavanones.

Overview

Flavanones are a kind of flavonoids, which refers to a class of compounds derived from 2-phenyl dihydrochromone as the parent nucleus. Most of the flavanones exist in plants are hydroxyl derivatives, and there may be methoxy or other substituents in the parent nucleus. Flavanones are generally colorless compounds, and molecules have chiral carbon atoms, so they have optical activity. There is a single bond between C2 and C3, and the carbonyl group at the C4 position is typical ketone. When treated with alkali, flavanone is easy to form chalcone through ring-opening reaction, and then turns into flavanone after acidification. The two are isomers of each other, and often coexist in plants at the same time. Since the biological activity of flavanones has been widely concerned, it is necessary to establish an efficient, rapid, accurate and scientific method for the analysis of flavanones. Ultrahigh performance liquid chromatography coupled with triple-quadrupole tandem mass spectrometry (UPLC-MS/MS) is a sensitive analytical technique that enables detection, identification and quantification of a variety of flavanones by characterizing their chemical structure, even at low abundance. Comprehensive phytohormone profiling helps elucidate a plant’s responses to different conditions such as mutations, stress, and hormone treatments.

Applications of Flavanones Analysis

  • Reveal mechanisms of plant development
  • Uncover plant's responses to biotic and abiotic stresses
  • Safety assessment of crops
  • Crop improvement

Advantages of Our Flavanones Analysis Service

Rich experience in plant sample handling and analysis of various flavanones

Enable analysis of flavanones present in a variety of plant species at cell/tissue level.

Accurate quantification of flavanones with UPLC-MS/MS

Flexible statistical analysis and bioinformatics analysis

Service Workflow

Since different tissues have different matrix properties, Creative Proteomics has developed multiple novel sample preparation techniques. Our services allow the profiling of multiple flavanones, including catechin.

Flavanones-Analysis-1.jpg

Analysis patterns: multi-response monitoring (MRM) mode under electrospray positive ion scanning

Linear: R>0.99

Time: dozens of minutes, slightly different according to different ingredients

Analysis content:

  • Standard curve creation
  • Raw data preprocessing
  • Absolute quantitation of phytohormones
  • Differential coumarins screening
  • Optimal analyses

List of Detectable Flavanones at Creative Proteomics

CatechinNaringeninFarrerol

Sample Requirements

1. Fresh plant tissues from leaf, flower, stem, root, seed, or fruit: >1 g. Provide young plant tissues for the best results. Samples should be frozen in liquid nitrogen immediately after collection, and then transferred to -80°C for storage.

2. Cells stored in -80°C.

At least 3 biological replicates.

Deliverables

  • Experimental procedure
  • Parameters of UPLC-MS/MS
  • Purity analysis report
  • UPLC-MS/MS raw data files and UPLC-MS/MS data quality checks
  • Metabolites quantification data
  • Custom analysis report

UPLC-MS/MS-based profiling of flavanones enables quantitative analyses of flavanones in a faster, convenient, and sensitive manner. With decades of experience in UPLC-MS/MS services, Creative Proteomics has a proven track record supporting diverse flavanones detection and quantification. We can meet your specific project requirements, from sampling to bioinformatics.

For Research Use Only. Not for use in diagnostic procedures.

Metabolomics Service
5%-20% Discount

Expires: Nov.15 — Dec.5, 2021

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