Flavones Analysis

Flavones are secondary metabolized polyphenolic compounds widely found in plants, mainly C6-C3-C6 compounds formed by bridging two benzene rings (A and B rings) with three carbon atoms. There are many types and numbers of flavones that have emerged. Generally, most flavones are combined with glycosides, giving them a more complex molecular structure. The chemical structure of flavones determines to some extent their diverse biological activities, and the biological activities vary with different structures. These compounds have been found to have antioxidant, antitumor, anticancer, antibacterial, immunomodulatory, and suppression of body dysfunction effects, as well as the advantages of low toxicity and high activity.

Due to their pharmacological effects, flavones are widely used in the pharmaceutical and food industries. However, their molecular structure leads to their poor solubility and limited bioavailability. In order to help deepen the study of flavones' mechanism of action and accelerate their research in pharmaceutical development and clinical applications, Creative Proteomics can provide you with high-resolution and reliable structural analysis of flavonoids as well as flavone identification and quantification using HPLC-MS/MS and HPLC-DAD-MS technologies.

Advantages of Our Flavones Analysis Service

• Rich experience in plant sample handling and analysis of various flavones
• Accurate quantification of flavones with HPLC-MS/MS and HPLC-DAD-MS
• Flexible statistical analysis and bioinformatics analysis

Service Workflow

Since different tissues have different matrix properties, Creative Proteomics has developed multiple novel sample preparation techniques. Our services allow the profiling of various flavones, including baicalein, baicalin, etc.

Linear range: r>0.99

Elution time: dozens of minutes, slightly different according to different ingredients

Analysis content:

• Standard curve creation
• Raw data preprocessing
• Absolute quantification of flavones
• Differential metabolites screening
• Optimal analyses

List of Detectable Flavones at Creative Proteomics

Sample Requirements

• Plant tissues: 200 mg/sample
• Animal tissues: 200 mg/sample
• Blood/plasma: 500 ul/sample
• Urine: 500 ul/sample
• Cells: 1x10⁷/sample

We can also accept other types of samples. Please contact our technical staff for specific testing requirements.

Deliverables

• Experimental procedure
• Parameters of HPLC-MS/MS and HPLC-DAD-MS
• Raw data files and data quality checks
• Metabolite quantification data
• Custom analysis report

With decades of experience in mass spectrometry services, Creative Proteomics has a proven track record supporting diverse flavones detection and quantification. We can meet your specific project requirements, from sampling to bioinformatics.