Baicalin Analysis Service

What is Baicalin?

Baicalin is a kind of flavonoid extracted and isolated from the dried root of scutellaria baicalensis georgi. Baicalin dissolves in alkali and ammonia and turns yellow at first, then turns black and brown soon. Baicalin has the effects of anti-tumor, anti-pathogen and anti-allergic reaction, and has a protective effect on liver injury, the improvement of diabetic nephropathy, the repair and protection of brain injury, and also has a certain effect on retinopathy. It can be used for bacteriostasis, diuresis, anti-inflammatory, cholesterol-lowering, anti-thrombosis, easing asthma, detoxification, hemostasis and anti-allergic reaction. Baicalin has a wide range of uses, and it is very important to establish an analytical method for baicalin in plant metabolites.

Creative Proteomics provides the simple, rapid, accurate, affordable and highly automated analytical method and equipment to analyse baicalin. High performance liquid chromatography with diode array detection and mass spectrometer (HPLC-DAD-MS) can solve some of the problems associated with baicalein analysis. Improved extraction and purification steps can also increase the sensitivity of the analytical method.

How Can Creative Proteomics Support Your Baicalin Analysis?

Understanding the pharmacokinetics, bioavailability, and metabolism of baicalin is an indispensable task, rooted in the need to develop drugs based on this particular compound. We used liquid chromatography-mass spectrometry (LC-MS) to identify, quantify and characterize baicalin and its metabolites in biological samples. Liquid chromatography-mass spectrometry proved to be highly efficient in separating baicalin from complex matrices (e.g. blood, urine, or tissue samples), in addition to detecting its molecular weight and fragmentation patterns, providing valuable structural and metabolic information.

The LC-MS analysis of baicalin is a multi-step process that starts with the extraction of the compound from biological samples by using a suitable solvent, followed by separation on a chromatographic column, and finally by mass spectrometry for detection and quantification.

Our experienced team of scientists has extensive expertise in the analysis of baicalin and other natural products using state-of-the-art LC-MS instrumentation and software. We offer a wide range of baicalin analysis services, including:

• Quantification of baicalin and its metabolites in biological samples
• Structural elucidation of baicalin and its derivatives
• Pharmacokinetic analysis of baicalin in animal and human samples
• Investigation of drug-drug interactions of baicalin with other compounds
• Customized analysis services tailored to the specific needs of our clients

Service Workflow

Our newly developed sample preprocessing method provides safety, environmental protection, rapidness, effectiveness, and the average recovery rate is over 99%. Sample preprocessing at Creative Proteomics complies with the in-house standard operation procedures (SOP), ensuring maximized homogeneity between samples.

Figure 1. Baicalin analysis service workflow.

Mass spectrometric conditions: electrospray ion source positive ion mode, multi-reaction monitoring mode data collection

Mobile phase: formic acid - water - acetonitrile

Linear range: r>0.99

Elution time: dozens of minutes, slightly different according to different ingredients

Analysis content:

• Standard curve creation
• Raw data preprocessing
• Absolute quantitation of baicalin
• Differential metabolites screening
• Optimal analyses

Sample Requirements

1. Fresh tissues: >1 g. If possible, samples should be frozen in liquid nitrogen right after collection, and then transferred to -80°C for storage.

2. Product samples.

At least 3 biological replicates.

Delivery

• Experimental procedure
• Parameters of HPLC-DAD-MS
• Purity analysis report
• HPLC-DAD-MS raw data files and HPLC-DAD-MS data quality checks
• Metabolites quantification data
• Custom analysis report

HPLC-DAD-MS based profiling of baicalin enables quantitative analyses in a faster, more convenient, and more sensitive manner. With decades of experience in HPLC-DAD-MS services, Creative Proteomics has a proven track record supporting diverse baicalin detection and quantitation. We can meet your specific project requirements, from sample to bioinformatics.