What are Plant Peptide Hormones?
Plant peptide hormones are crucial signaling molecules that orchestrate a myriad of biological processes in plants. These peptides regulate growth, development, and responses to environmental stimuli. Understanding their roles is fundamental for unraveling the complex mechanisms governing plant physiology.
Functions of Plant Peptide Hormones
Plant peptide hormones exert influence over several physiological aspects, including:
- Cell Growth and Division: Regulating cell expansion and division for proper plant development.
- Immune Responses: Activating defense mechanisms against pathogens.
- Root Development: Influencing root architecture and nutrient absorption.
- Stress Responses: Mediating responses to abiotic stresses such as drought, salinity, and temperature fluctuations.
Analyzing plant peptide hormones is imperative for gaining insights into the intricate signaling pathways that dictate plant behavior. It provides researchers and industries with the knowledge needed to enhance crop yield, optimize plant growth conditions, and develop resilient plant varieties. Creative Proteomics recognizes the significance of this analysis and has tailored services to meet the diverse needs of researchers and industry professionals.
Plant Peptide Hormone Analysis by Creative Proteomics
Peptide Identification: Harness the power of advanced mass spectrometry techniques to precisely identify and characterize plant peptide hormones within your samples.
Quantitative Analysis: Gain valuable insights into signaling dynamics through our quantitative analysis, providing accurate concentration levels for a deeper understanding of plant physiology.
Structural Analysis: Uncover the molecular secrets with our in-depth structural analysis, revealing peptide sequences, post-translational modifications, and three-dimensional structures.
Functional Studies: Explore the impact of plant peptide hormones on vital physiological processes, including growth, development, and stress responses, through our comprehensive functional studies.
Bioinformatics Analysis: Leverage our expertise in bioinformatics to interpret your data effectively, including pathway analysis, prediction of interacting partners, and a holistic assessment of cellular processes.
Customized Solutions: Recognizing the unique nature of each project, we offer tailored solutions for specific plant species or research objectives, ensuring alignment with your goals.
Data Interpretation and Reporting: Receive detailed reports with comprehensive data interpretation, empowering you with a clear understanding of your results and their implications for your ongoing research.
Plant Peptide Hormone Assay Platforms
Our plant peptide hormone assays leverage state-of-the-art mass spectrometry (MS) technologies, providing unparalleled accuracy and sensitivity in unraveling intricate signaling pathways. Explore the instruments driving our cutting-edge analysis:
Triple Quadrupole Mass Spectrometer (e.g., AB Sciex QTRAP 6500): Precision in Quantitation
The AB Sciex QTRAP 6500 ensures exceptional sensitivity and selectivity for quantitative analysis. It excels in accurately measuring plant peptide hormone concentrations, enabling a deeper understanding of their roles in cellular processes.
Orbitrap Mass Spectrometer (e.g., Thermo Scientific Q Exactive HF-X): High-Resolution Structural Analysis
The Thermo Scientific Q Exactive HF-X, equipped with Orbitrap technology, empowers our structural analysis. Uncover the intricate details of peptide sequences and modifications with unparalleled resolution, providing a comprehensive view of plant peptide hormone structures.
Time-of-Flight Mass Spectrometer (e.g., Waters Xevo G2-XS QTof): Comprehensive Peptide Profiling
The Waters Xevo G2-XS QTof excels in high-resolution analysis, facilitating detailed peptide profiling. Explore the diversity of plant peptide hormones with accuracy, allowing for a comprehensive assessment of their roles in biological processes.
NanoLC-MS/MS System (e.g., Thermo Scientific UltiMate 3000 RSLCnano): Enhanced Sensitivity in Peptide Identification
The Thermo Scientific UltiMate 3000 RSLCnano, coupled with MS/MS, enhances sensitivity in peptide identification. This platform excels in unraveling the complexity of plant peptide hormone mixtures, ensuring robust and reliable results.
List of Detectable Plant Peptide Hormone
Systemin | CLV3/ESR-related ('CLE') peptide family | ENOD40 | Phytosulfokine (PSK) |
POLARIS (PLS) | Rapid Alkalinization Factor (RALF) | SCR/SP11 | ROTUNDIFOLIA4/DEVIL1 (ROT4/DVL1) |
Inflorescence deficient in abscission (IDA) |
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Sample Requirements of Plant Peptide Hormone Analysis
Sample Type | Recommended Volume |
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Leaf Tissue | 100 mg (fresh weight) |
Root Tissue | 50 mg (fresh weight) |
Stem Tissue | 50 mg (fresh weight) |
Seed Extract | 20 mg (fresh weight) |
Floral Bud | 50 mg (fresh weight) |
Whole Plant Extract | 200 mg (fresh weight) |
Cell Cultures (Suspension) | 5 mL (cell pellet) |
Cell Cultures (Adherent) | 1 x 106 cells |
Xylem Sap | 1 mL |
Phloem Sap | 1 mL |
Root Exudates | 5 mL |
Rhizosphere Soil | 10 g |
Seeds | 20 seeds |
Pollen | 1 g (fresh weight) |
For Research Use Only. Not for use in diagnostic procedures.