Animal Untargeted Lipidomics Service

Overview

Lipidomics, a branch of metabolomics, is a large-scale study of all lipid molecules within a biological system including fluid cell and tissue. It is well documented that lipid molecules are the main structural components of biological membranes, which play important roles in the energy storage for living cells as well as mediation of cell signalling. The comprehensive identification and precise quantification of lipid molecules is important for understanding cellular physiology and pathophysiology¹. Rapid advances in high-performance liquid chromatography-mass spectrometry (HPLC-MS) technologies allow us for fast and sensitive detection of a wide variety of lipid species. One of the primary approaches undertaken in MS-based workflows is untargeted lipidomics. The untargeted lipidomics provides a powerful tool to assess medical risks, identify novel biomarkers and gain insight to molecular mechanisms of disease by analysing hundreds of lipid species in individual’s samples. The untargeted lipidomics analysis has been applied in wide fields including biomarker discovery; human disease research; clinical diagnostics; drug discovery; food safety; neonatal screening; nutrition and systems biology². With integrated array of separation, characterization, identification and quantification experience, Creative Proteomics provides reliable, rapid and cost-effective untargeted lipidomics services for your various scientific purposes.

The Main Applications of Untargeted Metabolomics Service

Biomarker discovery

Disease diagnostics

Drug discovery

Food safety

Metabolic disease research

Environmental health

Advantages of Our Untargeted Metabolomics Service

Rich experience in different types of animal sample preparation

State of art facilities

Professional experiment design and data analysis

Competitive price

Short turnaround time

Service Workflow

The animal untargeted lipidomics service provided by Creative Proteomics is based on our cutting-edge chromatographic separation and mass spectrometry platforms. The experimental workflow involves 4 steps: sample collection, lipid extraction, MS data analysis and bioinformatics analysis (Figure 1). Our service will be tailored to specific samples and needs for optimal results.

Figure 1. The overall workflow of animal untargeted lipidomics service (Adapted from Watson, A.D.)

List of Pl

Sample Requirements

We can analyze any biological materials including but not limited to cells and solid tissues  from humans and animals, such as mice, rats, rabbits.  If you need transport your samples to us, please follow the below requirements for different types of sample:

• Blood/plasma: 500ul/sample
• Urine: 1ml/sample
• Tissue: 200mg/sample
• Cells: 1x10⁷/sample
• Feces: 500mg/sample
• Shipment condition: dry ice

Report Delivery

• Experimental procedures
• Instrument parameters of liquid chromatography and mass spectrometry
• MS raw data files and the summary of mass spectrometry data quality
• The identification/quantification of lipidic molecules data
• Bioinformatics analysis report (PCA, KEGG, etc.)

Equipped with advanced mass spectrometry for the determination of lipid components, powerful bioinformatic analysis software and a panel of experienced technicians and scientists, we provide customer-tailored animal untargeted lipidomics analysis service with rapid experimental procedures and easy to read report, to advance your scientific research.

References

1. Watson, A.D. Lipidomics: a global approach to lipid analysis in biological systems. J. Lipid Res. 2006, 47: 2101-2011.
2. Shevchenko, A; Simons, KA. Lipidomics: coming to grips with lipid diversity. Nat Rev Mol Cell Biol. 2010, 11:593-598.