Plant Hormone Analysis

Overview

Plant hormones are highly bioactive signaling molecules that act as chemical messengers, mediating and controlling physiological processes during plant growth and development as well as stress responses by additive, synergistic, or antagonistic actions. Plant hormones are often present in trace amounts and their levels depend strongly on the plant organ, plant developmental stage, and environmental conditions. Plant hormones are structurally diverse active compounds that are divided into several major groups including auxins, cytokinins (CTK), gibberellins (GA), abscisic acid (ABA), ethylene (ETH), jasmonate (JA), salicylic acid (SA), strigolactone (SL) and brassinosteroids (BR). Mass spectrometry is a sensitive analytical technique that enables detection, identification and quantification of a variety of plant hormones by measuring their mass and characterizing their chemical structure, even at low abundance. MS instruments combine with either gas chromatography (GC) or liquid chromatography (LC) to effectively separate organic molecules from complex samples. Comprehensive phytohormone profiling helps elucidate a plant's responses to different conditions such as mutations, stress, and hormone treatments.

Applications of Plant Hormones Analysis

• Reveal mechanisms of plant development
• Uncover plant's responses to biotic and abiotic stresses
• Safety assessment of crops
• Crop improvement

Advantages of Our Plant Hormones Analysis Service

Rich experience in plant sample handling and analysis of various plant hormones

Enable analysis of plant hormones present in a variety of plant species at cell/tissue level

Accurate quantification of plant hormones with LC-MS and GC-MS

Flexible statistical analysis and bioinformatics analysis

Service Workflow

Since different tissues have different matrix properties, Creative Proteomics has developed multiple novel sample preparation techniques. Our services allow the profiling of more than 100 plant hormones, including auxins, GAs, CKs, ABA, BRs, JAs, SA, etc.

Mode: MRM

Precision: pmol

Linear: R2 > 0.99

Analysis content:

• Standard curve creation
• Raw data preprocessing
• Absolute quantification of phytohormones
• Differential metabolites screening
• Optimal analyses such as KEGG pathway analysis and hierarchical clustering

List of Detectable Plant Hormones at Creative Proteomics

Sample Requirements

1. Fresh plant tissues from leaf, flower, stem, root, seed, or fruit: >1 g. Provide young plant tissues for the best results. Samples should be frozen in liquid nitrogen immediately after collection, and then transferred to -80°C for storage.

2. Cells stored in -80°C.

At least 3 biological replicates.

Deliverables

• Experimental procedure
• Parameters of liquid chromatography and MS
• MS raw data files and MS data quality checks
• Metabolites quantification data
• Custom analysis report

Mass spectrometry-based profiling of plant hormones enables quantitative analyses of plant hormones in a faster, convenient, and sensitive manner. With decades of experience in mass spectrometry services, Creative Proteomics has a proven track record supporting diverse plant hormone detection and quantification. We can meet your specific project requirements, from sampling to bioinformatics.

References

1. Ondřej Novák, Richard Napier, Karin Ljung. Zooming In on Plant Hormone Analysis: Tissue- and Cell-Specific Approaches. Annu Rev Plant Biol, 2017 Apr 28(68):323-348.
2. Xiangqing Pan, Xuemin Wang. Profiling of plant hormones by mass spectrometry. Journal of Chromatography B Analytical Technologies in the Biomedical & Life ences, 2009, 877(26):2806-2813.