Metabolomics Creative Proteomics

Creative Proteomics Metabolomics

Strigolactone (SL) Analysis Service

Strigolactone (SL) Analysis Service

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Powered by LC-MS/MS systems in MRM mode, our strigolactone analysis service offers a qualitative and quantitative determination of SLs, providing insights into their roles in developmental biology and plant physiology, and the possibility of harnessing the benefits of strigolactones for sustainable agriculture.

Overview

Strigolactones (SLs) are carotenoid derivatives that belonged to terpene lactones, a family of active compounds first found in leaf extracts of Ginkgo biloba. SLs were accepted as a new class of plant hormones in 2008. More than 25 types of SLs have been identified in various plant species and they occur at extremely low concentrations in the root exudates of plants. SLs are responsible for regulating plant organ development and fruit development and play important roles in the response to various biotic and abiotic stresses. SLs are involved in symbioses between plants and mycorrhizal fungi and speed up the germination of parasitic weeds like Orobanche and Striga species. However, SLs are a poorly characterized type of phytohormone since the low concentration of SLs inhibits SL analysis. Liquid chromatography-tandem mass spectrometry (LC-MS) emerges as the most popular technique that combines high-resolution chromatography with sensitive mass spectrometric detection. Compared with gas chromatography-MS (GC-MS), LC-MS/MS method omits the step of derivatization, thus more efficient and reliable.

Model of SL signaling and downstream physiological and phenotypic effectsFigure 1. Model of SL signaling and downstream physiological and phenotypic effects (Aliche E.B. et al. 2020).

Applications of SL Analysis

  • Elucidate mechanisms of SL biosynthesis
  • Clarify SL perception and signal transduction
  • Reveal downstream physiological and phenotypic effects of SLs
  • Sustainable agriculture

Advantages of Our SL Analysis Service

Improved sample preprocessing ensures high recovery rates and effectiveness

Qualitative and quantitative chemical analysis of 5-DS

Customizable protocols and data analyses

End-to-end, high-resolution SL analysis with comparable price

Service Workflow

We utilize the most advanced separation method, ultra-high performance liquid chromatography (UHPLC) coupled with mass spectrometry (MS) for SL analysis, to offer great separation efficiencies, sensitive detection, and short analysis time. LC-MS/MS in multiple-reaction monitoring (MRM) is employed for data acquisition. Our team of experts is experienced in handling a variety of samples, and our sample preprocessing method is proved to be safe, environmentally friendly, effective, and ensures consistently high recovery rates and maximum homogeneity between samples.

Service Workflow

Quantification method: external reference method

Mode: MRM, capable of simultaneously detecting more than 1000 MRM ion pairs

Precision: ≤10-9 g

Positive/Negative polarity switching time: ≤ 20 ms, allowing for the acquisition of Q1/Q3 MRM transition mass spectra in both ionization modes from a single LC-MS/MS run.

Analysis content:

  • Standard curve creation
  • Raw data preprocessing
  • Absolute quantification of SLs
  • Differential metabolites screening
  • Optimal analyses such as KEGG pathway analysis and hierarchical clustering

List of Detectable SLs at Creative Proteomics

Detectable SLsCASQuantification Method
5-deoxystrigol (5DS)151716-18-6External Reference method

Sample Requirements

Root exudates or tissue: >2 g. Provide fresh samples for best results. Samples should be frozen in liquid nitrogen immediately after collection, and then transferred to -80°C for storage.

At least 3 biological replicates.

Deliverable

  • Experimental procedure
  • Parameters of liquid chromatography and MS
  • Extracted mitochondria and purified protein sample
  • Purity analysis report
  • MS raw data files and MS data quality checks
  • 5-DS quantification data
  • Custom data analysis report

Creative Proteomics provides numerous plant hormone analyses based on state-of-the-art GC-MS/MS or LC-MS/MS systems. We are committed to clarifying the biosynthesis, signaling, action, and perception of a wide range of phytohormones.

References

  1. Halouzka R, Zeljković S Ć, Klejdus B, et al. Analytical methods in strigolactone research. Plant Methods, 2020, 16: 1-13.
  2. Wu H, Li H, Chen H, et al. Identification and expression analysis of strigolactone biosynthetic and signaling genes reveal strigolactones are involved in fruit development of the woodland strawberry (Fragaria vesca). BMC plant biology, 2019, 19(1): 1-19.
  3. Seto Y, Yasui R, Kameoka H, et al. Strigolactone perception and deactivation by a hydrolase receptor DWARF14. Nature communications, 2019, 10(1): 1-10.
  4. Aliche E B, Screpanti C, De Mesmaeker A, et al. Science and application of strigolactones. New Phytologist, 2020, 227(4): 1001-1011.
For Research Use Only. Not for use in diagnostic procedures.

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