Cofactors and Vitamins Analysis Service

Overview

A cofactor is a kind of nonprotein chemical compound that maintains various enzymes' proper functioning. There are two types of cofactors: inorganic ions [e.g., Calcium or Magnesium ions] and organic molecules. Most cofactors are vitamins or are derived from vitamins. Vitamins are organic compounds that are essential in very small amounts for the maintenance of normal function in animals. They normally cannot be synthesized at adequate levels by the body and have to be obtained from the diet. cofactors play an important role in the metabolism of all organisms as reflected by the wide variety of chemical reactions in which they participate. Manipulation in use of cofactors is critical in metabolic engineering. In this process, the concentrations of cofactors are changed in order to maximize or minimize metabolic fluxes. It can be used to optimize the production of metabolite products to increase the efficiency of metabolic networks. Their abnormalities are closely associated to various human pathologies like diabetes and cancer. Scientists at Creative Proteomics take advantage of highly quantitative methods with high-performance liquid chromatography (HPLC) for the determination of metabolism of cofactors and vitamins metabolism related metabolites levels in various samples. Furthermore, we provide reliable, rapid and cost-effective metabolism of cofactors and vitamins related service to speed up your research aims ranging from diseases diagnosis to gaining new insights in disease mechanism or treatment.

The Main Applications of Cofactors and Vitamins Analysis Service

• Identifying novel biomarkers of diseases
• Improving diagnose diseases
• Accelerating development of novel drugs
• Gaining new mechanism of diseases

Advantages of Our Cofactors and Vitamins Analysis Service

• Constantly optimized protocol and analytical software
• Professional experiment design
• Quick turnaround time
• High accuracy, specificity, and sensitivity

Service Workflow

Creative Proteomics provides metabolism of cofactors and vitamins service in a reliable and effective manner, based on our cutting-edge high-performance liquid chromatography- mass spectrometry (HPLC-MS) platforms. The experimental procedures contain four main procedures: sample collection, metabolites extraction, HPLC-MS data analysis and bioinformatics analysis (Figure 1). Our service will be tailored to specific samples and needs for optimal results.

Figure 1. The overall workflow of metabolism of cofactors and vitamins

List of Partial Detectable Metabolites at Creative Proteomics

Sample Requirements

We can analyze a wide range of biological materials including but not limited to cells and solid tissues from animals. If you need transport your samples to us, please follow the following requirements for different types of sample:

• Blood/plasma: 500ul/sample
• Urine: 1ml/sample
• Tissue: 200mg/sample
• Cells: 1x10⁷/sample
• Feces: 500mg/sample
  Shipment condition: dry ice

Report Delivery

• Experimental protocols
• Instrumental factors of HPLC
• The raw data files of HPLC and the summary of HPLC data quality
• Bioinformatics analysis report

Based on advanced HPLC platforms for the determination of metabolism of cofactors and vitamins related metabolites, professional bioinformatic analysis software and experienced technicians and scientists, Creative Proteomics provides customer-tailored metabolism of cofactors and vitamins analysis service with rapid experimental procedures and easy to read report, to accelerate your scientific research.

References

1. Wang Yipeng, San KaYiu, Bennett George N. Cofactor engineering for advancing chemical biotechnology. Curr Opin Biotechnol. 2013.24:994-999.
2. Valdés-Ramos Roxana, Guadarrama-López Ana Laura, Martínez-Carrillo Beatriz Elina. et al. Endocr Metab Immune Disord Drug Targets. 2015.15:54-63.