Metabolomics Creative Proteomics

Creative Proteomics Metabolomics

Biotin Metabolism Service

Biotin Metabolism Service

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Creative Proteomics is a pioneer in metabolic analysis service and provides reliable, professional and cost-effective biotin metabolism analysis service in animal. We leverage our rich experience, professional expertise, and advanced technologies to speed up your scientific purposes of biotin metabolism analysis in animal.

Overview

Biotin is a vitamin that is an essential nutrient for animals. As a cofactor for enzymes involved in carboxylation reactions, there are five biotin-dependent carboxylases: methylcrotonyl-CoA carboxylase, pyruvate carboxylase, propionyl-CoA carboxylase and two forms of acetyl-CoA carboxylase. These enzymes catalyse key carboxylation reactions in gluconeogenesis, fatty acid metabolism and amino acid catabolism. Thus, biotin plays an essential role in maintaining metabolic homeostasis. Mammals cannot synthesize biotin in vivo and therefore must obtain it from material synthesized by intestinal microflora and from dietary sources. Two principal pathways of biotin catabolism have been identified in mammals (Figure 1). In the first pathway, the valeric acid side chain of biotin is degraded by β-oxidation. β-Oxidation of biotin leads to the formation of bisnorbiotin, tetranorbiotin, and related intermediates that are known to result from β-oxidation of fatty acids. The cellular site of this β-oxidation of biotin is uncertain. Spontaneous decarboxylation of the unstable β-keto acids causes formation of bisnorbiotin methyl ketone and tetranorbiotin methyl ketone. The aberrant biotin metabolism is closely connected to various human diseases. Scientists at Creative Proteomics take advantage of highly quantitative methods with high-performance liquid chromatography (HPLC) for the determination of biotin metabolism related metabolites levels in various samples. Furthermore, we provide reliable, rapid and cost-effective biotin metabolism related service to speed up your research aims ranging from diseases diagnosis to gaining new insights in disease mechanism or treatment.

The overview of biotin catabolismFigure 1. The overview of biotin catabolism

The Main Applications of Biotin Metabolism Service

  • Identifying novel biomarkers of diseases
  • Improving diagnose diseases
  • Accelerating development of novel drugs
  • Gaining new mechanism of diseases

Advantages of Our Biotin Metabolism Service

  • Constantly optimized protocol and analytical software
  • Professional experiment design
  • Quick turnaround time
  • High accuracy, specificity, and sensitivity.

Service Workflow

Creative Proteomics provides biotin metabolism analysis service in a reliable and effective manner, based on our cutting-edge HPLC platforms. The experimental procedures contain four main procedures: sample collection, metabolites extraction, HPLC data analysis and bioinformatics analysis (Figure 2). Our service will be tailored to specific samples and needs for optimal results.

The overall workflow of biotin metabolismFigure 2. The overall workflow of biotin metabolism

List of Partial Detectable Biotin Metabolism Related Metabolites at Creative Proteomics

Pimelic acid7,8-DiaminononanoateBiotinyl-5'-AMPBiotin sulfoxide
6-Carboxyhexanoyl-CoADethiobiotinBiotinyl-CoABiotin sulfone
8-Amino-7-oxononanoateBiotinBisnorbiotinTetranorbiotin

Sample Requirements

We can analyze a wide range of biological materials including but not limited to cells and solid tissues from animals. If you need transport your samples to us, please follow the following requirements for different types of sample:

  • Blood/plasma: 500ul/sample
  • Urine: 1ml/sample
  • Tissue: 200mg/sample
  • Cells: 1x107/sample
  • Feces: 500mg/sample

Shipment condition: dry ice

Report Delivery

  • Experimental protocols
  • Instrumental factors of HPLC
  • The raw data files of HPLC and the summary of HPLC data quality
  • Bioinformatics analysis report

Based on advanced HPLC platforms for the determination of biotin metabolism related metabolites, professional bioinformatic analysis software and experienced technicians and scientists, Creative Proteomics provides customer-tailored biotin metabolism analysis service with rapid experimental procedures and easy to read report, to accelerate your scientific research.

References

  1. Galluzzi L, Vacchelli E, Michels J.  et al. Effects of vitamin B6 metabolism on oncogenesis, tumor progression and therapeutic responses.Oncogene. 2013. 32:4995-5004.
  2. Pacheco-Alvarez Diana, Solórzano-Vargas R Sergio, Río Alfonso León Del. Biotin in metabolism and its relationship to human disease. Arch Med Res. 2002.33:439-447.
For Research Use Only. Not for use in diagnostic procedures.

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