Metabolomics Creative Proteomics

Creative Proteomics Metabolomics

Biotin Metabolism Service

Biotin Metabolism Service

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Overview

Biotin (Vitamin B7) is a vitamin essential for the maintenance of normal physiological functions in the animal organism. It plays an important role in the metabolism of glucose, amino acids and fatty acids in mammals as a component of four carboxylase coenzymes. There are five biotin-dependent carboxylases: methylcrotonyl-CoA carboxylase, pyruvate carboxylase, propionyl-CoA carboxylase and two forms of acetyl-CoA carboxylase. These enzymes catalyse key carboxylation reactions in gluconeogenesis, fatty acid metabolism and amino acid catabolism. Thus, biotin plays an essential role in maintaining metabolic homeostasis. Mammals cannot synthesize biotin in vivo and therefore must obtain it from material synthesized by intestinal microflora and from dietary sources. Two principal pathways of biotin catabolism have been identified in mammals (Figure 1). In the first pathway, the valeric acid side chain of biotin is degraded by β-oxidation. β-Oxidation of biotin leads to the formation of bisnorbiotin, tetranorbiotin, and related intermediates that are known to result from β-oxidation of fatty acids. The cellular site of this β-oxidation of biotin is uncertain. Spontaneous decarboxylation of the unstable β-keto acids causes formation of bisnorbiotin methyl ketone and tetranorbiotin methyl ketone. The aberrant biotin metabolism is closely connected to various human diseases.

Creative Proteomics has the LC-MS technology platform to provide you with qualitative and quantitative biotin analysis to accelerate your project.

The overview of biotin catabolismFigure 1. The overview of biotin catabolism

Advantages of Our Biotin Metabolism Service

  • Constantly optimized protocol and analytical software
  • Professional experiment design
  • Quick turnaround time
  • High accuracy, specificity, and sensitivity.

Service Workflow

Creative Proteomics provides biotin metabolism analysis service in a reliable and effective manner, based on our cutting-edge HPLC-MS platforms. The experimental procedures contain four main procedures: sample collection, metabolites extraction, HPLC-MS data analysis and bioinformatics analysis (Figure 2). Our service will be tailored to specific samples and needs for optimal results.

The overall workflow of biotin metabolism

List of Partial Detectable Biotin Related Metabolites at Creative Proteomics

Pimelic acid7,8-DiaminononanoateBiotinyl-5'-AMPBiotin sulfoxide
6-Carboxyhexanoyl-CoADethiobiotinBiotinyl-CoABiotin sulfone
8-Amino-7-oxononanoateBiotinBisnorbiotinTetranorbiotin

Sample Requirements

  • Plant tissues: 400mg/sample
  • Animal tissues: 200mg/sample
  • Blood/plasma: 500ul/sample
  • Urine: 1ml/sample
  • Cells: 1x107/sample
  • Feces: 500mg/sample

Shipment condition: dry ice

Report Delivery

  • Experimental procedures
  • The parameters of liquid chromatograph and mass spectrometer
  • HPLC and MS raw data files
  • Metabolite quantitative data

References

  1. Galluzzi L, Vacchelli E, Michels J. et al. Effects of vitamin B6 metabolism on oncogenesis, tumor progression and therapeutic responses.Oncogene. 2013. 32:4995-5004.
  2. Pacheco-Alvarez Diana, Solórzano-Vargas R Sergio, Río Alfonso León Del. Biotin in metabolism and its relationship to human disease. Arch Med Res. 2002.33:439-447.
For Research Use Only. Not for use in diagnostic procedures.

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