What is S-Adenosylmethionine?
S-Adenosylmethionine, or SAMe, is a vital molecule with a pivotal role in various cellular processes. Classified as a sulfur-containing coenzyme, SAMe is derived from the essential amino acid methionine and adenosine triphosphate (ATP). Its unique molecular structure encompasses an adenosine unit, a methionine moiety, and a sulfonium group, endowing it with the ability to donate methyl groups.
SAMe is primarily recognized as a key player in methylation reactions. Acting as a methyl donor, it participates in the modification and regulation of DNA, proteins, and lipids. This fundamental process is integral to cellular function and regulation. Beyond its role in methylation, SAMe serves as a precursor for neurotransmitter synthesis in the central nervous system. Neurotransmitters such as dopamine, serotonin, and norepinephrine, crucial for mood regulation and neurological function, trace their origins back to SAMe.
SAMe's influence extends to the epigenetic regulation of gene expression through DNA methylation. By adding methyl groups to specific DNA regions, SAMe plays a crucial role in cellular differentiation and development.
Analyzing SAMe is imperative for gaining insights into its quantitative and qualitative aspects. Researchers and clinicians utilize S-Adenosylmethionine analysis to understand cellular methylation dynamics, advancing fields such as cancer research, neurobiology, and epigenetics. Creative Proteomics offers a comprehensive solution for precise S-Adenosylmethionine analysis.
S-Adenosylmethionine Analysis Services Offered by Creative Proteomics
Quantitative Analysis: At Creative Proteomics, we specialize in delivering precise quantification of SAMe levels across diverse sample types. Our commitment lies in providing accurate measurements through advanced analytical techniques, empowering researchers and clinicians with dependable quantitative insights into SAMe within various biological contexts.
Qualitative Analysis: Moving beyond mere quantification, our qualitative analysis focuses on the detailed identification and characterization of SAMe and its associated compounds. This service offers a grounded understanding of the molecular landscape under investigation, facilitating a nuanced comprehension of SAMe's presence and interactions.
Customized Solutions: Recognizing the unique needs of our clients, Creative Proteomics offers tailored analytical solutions. Our customized approaches are designed to meet specific research or clinical requirements, ensuring practical flexibility and relevance in addressing distinct challenges faced by our valued partners.
S-Adenosylmethionine Analytical Techniques
| Analytical Technique | Description |
|---|
| Mass Spectrometry (MS) |
|
| - High-Resolution MS | Achieves exceptional mass accuracy and resolution for detailed analysis of SAMe. |
| - Liquid Chromatography-Mass Spectrometry (LC-MS) | Enhances separation, specificity, and sensitivity in SAMe analysis, enabling precise identification and quantification. |
| - Gas Chromatography-Mass Spectrometry (GC-MS) | Utilized for volatile compound analysis, enhancing capabilities through certain derivatization processes. |
| - Tandem Mass Spectrometry (MS/MS) | Involves isolating and fragmenting SAMe ions, providing structural information for enhanced compound identification. |
| Chromatographic Techniques |
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| - High-Performance Liquid Chromatography (HPLC) | Robust technique for separation and quantification of SAMe, ensuring accuracy through precise sample handling and column selection. |
| Nuclear Magnetic Resonance (NMR) |
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| - High-Resolution NMR | Studies the structure and dynamics of SAMe, offering insights into molecular interactions and aiding in characterization. |
| Sample Preparation Techniques |
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| - Solid-Phase Extraction | Used for purification and concentration of SAMe from complex samples, improving sensitivity and accuracy in downstream analysis. |
| - Derivatization | In specific cases, modifies SAMe's chemical structure to improve detectability for certain analytical techniques. |
Sample Requirements for S-Adenosylmethionine Assay
| Sample Type | Recommended Sample Volume |
|---|
| Biological Tissues (e.g., liver, kidney) | 50 - 100 mg |
| Cell Cultures (cell pellets or culture medium) | 1 x 106 - 1 x 107 cells |
| Serum/Plasma | 100 - 500 μL |
| Urine | 1 - 5 mL |
| Other Biological Fluids (CSF, pleural fluid, synovial fluid, etc.) | 100 - 500 μL |
| Saliva | 0.5 - 1 mL |
| Hair | 10 - 20 mg |
| Nail Clippings | 10 - 20 mg |
| Feces | 1 - 5 g |
For Research Use Only. Not for use in diagnostic procedures.
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