Overview
Tears not only moisten the eyeballs and produce immunity and defense, but also contain a lot of molecular information related to human physiology and pathology. The ingestion of therapeutic drugs and drugs of abuse can also be excreted through lacrimal secretion. In addition, because the eyes are directly exposed to the air, volatiles, aerosols and soluble particles in the air may also enter the eyes and be transferred to the tears. Due to the small secretion volume, complex composition, high salinity, and low metabolite concentration of tear fluid, the collection and analysis of trace tear fluid is extremely challenging.
According to different analysis needs, Creative Proteomics provides LC-MS-based analysis services for tear samples to help you analyze the target metabolites or the full spectrum of metabolism in the sample.
What We Can Provide:
Untargeted metabolomics analysis:
- Metabolic profile analysis
- Screening for differential metabolites
- Metabolic pathway analysis of differential metabolites
- Discover new biomarkers
Fig 1. The workflow of untargeted metabolomics analysis
Targeted metabolomics analysis:
- Mode: SRM / MRM
- Analysis content: Standard curve creation. Raw data preprocessing. Absolute quantification of metabolites.
Fig 2. The workflow of target metabolomics analysis
Sample Requirements
The collection of human tears involves strict safety requirements. Please contact our technical staff for specific requirements.
Quick-freeze in liquid nitrogen and store at -80°C, and send it on dry ice.
Deliverables
- Experimental procedure
- Parameters of liquid chromatography / gas chromatography and MS
- MS raw data files and MS data quality checks
- Bioinformatics analysis report
Turnaround time: 1-4 weeks
Bioinformatics Analysis
| Standard analysis | 1. Data quality control (QC correlation analysis, PCA analysis of total samples) |
| 2. Qualitative and quantitative results of metabolites |
| 3. Differential metabolite screening (PCA, PLS-DA dimensionality reduction treatment, volcano map) |
| 4. Differential metabolite analysis: Venn diagram, cluster heat map, metabolite correlation analysis, Z-score analysis, pathway analysis, ROC analysis |
| Personalized analysis | Weighted gene co-expression network analysis (WGCNA) |
| One way ANOVA |
Creative Proteomics provides metabolomics services for tear samples, and will deliver accurate and detailed data and analysis reports to you. If you want to detect other metabolites but have not found them, you can tell us through the inquiry form, and our technicians will communicate with you.
For Research Use Only. Not for use in diagnostic procedures.
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