Metabolomics Creative Proteomics

Creative Proteomics Metabolomics

Amino Acids Analysis

Amino Acids Analysis

Online Inquiry

Amino acids are biologically important organic compounds, consisting of amino (-NH2) and carboxyl (-COOH) functional groups, as well as side chains connected to each amino acid. They are not only the raw material for protein synthesis but also participate in the regulation of various physiological functions of cells, which is essential for maintaining life. Amino acids can be classified into aliphatic amino acids, aromatic amino acids, heterocyclic amino acids, acyclic amino acids, basic amino acids, sulfur-containing amino acids, hydroxyl amino acids, iodine-containing amino acids, etc., according to their different binding groups.

Creative Proteomics developed an amino acid analysis method based on UPLC-MS platform for absolute quantitative detection of 130+ amino acids and their derivatives.

Metabolic Pathways Analysis

  • Amino acids
  • Monoamines
  • Polyamines
  • Cholamines
  • Neurotransmitters
  • Cytotoxity markers
  • Immunogical biomarkers
  • Food consumption biomarkers

Advantages of Our Amino Acids Analysis Service

  • Rich experience in sample handling and analysis of more than 130 amino acids
  • The ability to analyze amino acids present in a variety of species at the cellular/tissue level
  • Efficient quantification of amino acids with LC-MS, UPLC-MS and GC-MS technology
  • Flexible statistical analysis and bioinformatics analysis

Service Workflow

To detect more than 130+ amino acids and their derivatives, we follow the basic process shown below. At the same time, we will adjust the experimental method according to different natures of samples, since Creative Proteomics has developed multiple novel sample preparation techniques.

Glycine analysis service workflow.

Mode: MRM
Precision: pmol
Linear: R2 > 0.99

Analysis content:

  • Standard curve creation
  • Raw data preprocessing
  • Absolute quantification of amino acids and their derivatives
  • Differential metabolites screening

The Amino Acids We Can Analyze Include But Are Not Limited To

Sample Requirements

  • Tissue: ≥10 mg
  • Cell: ≥1×106
  • Plasma/serum: ≥10 µL
  • Urine: ≥10 µL
  • Plant: ≥20 mg
  • Feces: ≥10 mg
  • DBS: ≥10 µL
  • Cell medium: ≥ 100 µL

Deliverables

  • Experimental procedure
  • Parameters of liquid chromatography and MS
  • MS raw data files and MS data quality checks
  • Metabolites quantification data
  • Custom analysis report

References

  1. Alseekh S, Fernie AR: Using Metabolomics to Assist Plant Breeding. In: Crop Breeding: Genetic Improvement Methods. Edited by Tripodi P. New York, NY: Springer US; 2021: 33-46.
  2. Tyler MI: Amino Acid Analysis. In: Amino Acid Analysis Protocols. Edited by Cooper C, Packer N, Williams K. Totowa, NJ: Humana Press; 2000: 1-7.
For Research Use Only. Not for use in diagnostic procedures.

Connect with Creative Proteomics

Contact UsContact Us
Verification Code
return-top