Metabolomics Creative Proteomics

Creative Proteomics Metabolomics

Proline Analysis Service

Proline Analysis Service

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Creative Proteomics provides an advanced technology platform, including high-performance liquid chromatography/gas chromatography technologies combined with mass-spectrometric technique, to accurately and efficiently analyze and quantify proline content in plants. This high-throughput approach is helpful to further explore the applications of proline in agriculture and plant development.

Overview

There are three forms of proline DL-Proline, L-Proline, and D-Proline. The commonly referred to proline is L-proline, a naturally occurring amino acid. L-Proline is found in almost all proteins and it is abundant in the body, second only to glutamine and alanine. Proline plays an important regulatory role in protein synthesis, metabolism of the body, wound healing, antioxidant and immune response.

Hydroxyproline is found in many plant proteins and is particularly associated with cell wall formation. Plant bodies often have a significant accumulation of proline under a variety of adversities such as drought, high temperature, low temperature, and salinity. In addition, proline has a wide range of applications in clinical, biomaterials, and industrial applications.

GC-MS and LC-MS with high resolution and sensitivity can be used to separate complex sample mixtures for the detection and quantification of specific amino acids. Creative Proteomics offers an advanced LC-MS technology platform for accurate and efficient analysis and quantification of proline content. In addition, we offer other amino acid and metabolite assays to meet your different needs.

(S)-Proline(left) and (R)-Proline (right) in zwitterionic form at neutral pHFigure 1. (S)-Proline(left) and (R)-Proline (right) in zwitterionic form at neutral pH

Advantages of Our Prolines Analysis Service

  • Comprehensive and unified service platform
  • Experienced sample extraction and data analysis team
  • MS-based platforms for accurate detection and quantification of prolines
  • Flexible statistical analysis
  • High repeatability and low-cost tests

Types of Detectable Substances

DL-ProlineL-ProlineD-ProlineHydroxyproline

Service Workflow

Proline analysis service workflow. Figure 2. Proline analysis service workflow.

Quantification methods: external reference method or isotope-labeled internal standard method

Mode: MRM, capable of simultaneously detecting more than 1000 MRM ion pairs

Precision: ≤10-9g

Positive/Negative polarity switching time: ≤20ms,allowing for the acquisition of Q1/Q3 MRM transition mass spectra in both ionization modes from a single LC-MS/MS run.

Analysis content:

  • Standard curve drawing
  • Raw data preprocessing
  • Absolute quantitation of prolines
  • Differential metabolites screening
  • Optimal analyses such as KEGG pathway analysis and hierarchical clustering

Sample Requirements

  • Plasma ≥ 200 μL/sample
  • Blood ≥ 200 μL/sample
  • Animal tissues ≥ 0.2 g/sample
  • Plant tissues (from leaf, flower, stem, root, or fruit) ≥ 1 g/sample.
  • Food products
  • Drugs
  • Others

We also accept other samples. Our technicians will communicate with you about the specific sample requirements.

Deliverables

  • Experimental procedure
  • Instrument parameters
  • MS raw data files and MS data quality checks
  • Metabolites quantification data
  • Custom analysis report

We can provide customers with sample processing, testing, analysis, and other professional technical services, and work with our customers to improve product quality.From sampling to analysis, we provide one-stop services. Our experienced team guarantees to produce credible results at a competitive price.

References

  1. Verbruggen N, Hermans C: Proline accumulation in plants: a review. AMINO ACIDS2008, 35(4):753-759.
  2. Ábrahám E, Rigó G, Székely G, Nagy R, Koncz C, Szabados L: Light-dependent induction of proline biosynthesis by abscisic acid and salt stress is inhibited by brassinosteroid in Arabidopsis. PLANT MOL BIOL2003, 51(3):363-372.
For Research Use Only. Not for use in diagnostic procedures.
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