Beta-Alanine Analysis Service

Overview

β-Alanine is a precursor for the synthesis of muscle-derived active peptides such as myostatin and goose myostatin. β-alanine is a dipeptide concentrated in muscle and brain tissue. β-alanine has been widely used in clinical nutrition as a sports nutrition supplement to enhance muscle endurance. β-alanine can improve animal performance, regulate muscle growth and myogenic active peptide content, and improve meat quality.

Figure 1. Comparison of β-alanine (right) and L-alanine (left)

In addition to the general importance of β-alanine (as a precursor of CoA) in the synthesis of phospholipids, the synthesis and degradation of fatty acids, and the operation of the tricarboxylic acid cycle, β-alanine is involved in secondary plant metabolism, including lignin biosynthesis. In addition, β-alanine is involved in a variety of stress responses in plants. β-alanine metabolism involves a variety of pathways and roles, such as nitrogen use efficiency, response to hypoxia, osmoprotection, vitamin B5, and CoA metabolism. Creative Proteomics provides efficient, advanced, and cost-effective mass spectrometry services to detect and quantify β-alanine.

Applications of β-Alanine Analysis

• Evaluation of plant growth
• Prediction of plant metabolism
• Plant disease diagnosis
• Crop improvement

Advantages of Our β-Alanine Analysis Services

• Output data at once for multiple samples
• Distinguish β-alanine from other amino acids
• β-Alanine content detection
• Provide data sorting and quick analysis
• Reasonable price

Service Workflow

Tissue or cell samples will first undergo pretreatment. β-Alanine will be separated from other amino acids, and then compared and analyzed efficiently.

Figure 2. Β-alanine analysis service workflow.

Quantitation methods: external reference method or isotope-labeled internal standard method

Mode: MRM, capable of simultaneously detecting more than 1000 MRM ion pairs

Precision: ≤10^-9 g

Positive/Negative polarity switching time: ≤20ms, allowing for the acquisition of Q1/Q3 MRM transition mass spectra in both ionization modes from a single LC-MS/MS run.

Analysis content:

• Standard curve creation
• Raw data preprocessing
• Absolute quantification of β-alanine
• Differential metabolites screening
• Optimal analyses such as KEGG pathway analysis and hierarchical clustering

Sample Requirements

• Tissue: 200 mg/sample
• Plasma ≥ 200 μL/sample
• Blood ≥ 200 μL/sample
• Drugs ≥ 0.2 g/sample

Deliverables

• Experimental procedure
• Parameters of liquid chromatography and MS
• HPLC-MS/MS raw data files and HPLC-MS/MS data quality checks
• Metabolites quantification data
• Custom analysis report

Creative Proteomics provides powerful analysis to detect β-alanine content and delivers credible references for your research. We use our rich experience and a systematic technology platform to meet your needs at any time.

References

1. https://encyclopedia.thefreedictionary.com/Beta-alanine
2. Vaughan RA, Gannon NP, Garcia-Smith R, Licon-Munoz Y, Barberena MA, Bisoffi M, Trujillo KA: beta-alanine suppresses malignant breast epithelial cell aggressiveness through alterations in metabolism and cellular acidity in vitro. MOL CANCER 2014, 13:14.
3. Hill CA, Harris RC, Kim HJ, Harris BD, Sale C, Boobis LH, Kim CK, Wise JA: Influence of β-alanine supplementation on skeletal muscle carnosine concentrations and high intensity cycling capacity. AMINO ACIDS 2007, 32(2):225-233.