Saponins Analysis

Overview

Saponins are plant glycosides that can form aqueous or colloidal solutions and soap-like foam. Saponins consist of saponin, sugar and uronic acid or other organic acid. According to the known molecular structure of saponin, saponins can be divided into two categories, one is steroid saponins, and the other is triterpenoid saponins. Saponins are mostly white or milky amorphous powder, a few are crystal, and have irritation to mucosa. Saponins are generally soluble in water, methanol and dilute ethanol, very soluble in hot water, hot methanol and hot ethanol, insoluble in ether, chloroform and benzene. Ginsenoside is the most effective medicinal ingredient of ginseng. There are nearly 30 kinds of ginsenoside, and each kind of ginsenoside has its specific pharmacological function. Soyasaponins have many beneficial physiological functions on human body. In addition to being used as medicine, soyasaponins can also be used in chemical industry as advanced cosmetics, food additives and surfactants. Mass spectrometry is a sensitive analytical technique that enables detection, identification and quantification of a variety of saponins by measuring their mass and characterizing their chemical structure, even at low abundance. MS instruments combine with either high performance liquid chromatography (HPLC) or electrospray ionization (ESI) to effectively separate organic molecules from complex samples.

Applications of Saponins Analysis

• Qualitative analysis of the main types of saponins
• Quantitative analysis of saponins content
• Ensure safety and quality
• Provide basis for improvement

Advantages of Our Saponins Analysis Service

Excellent saponins qualitative and quantitative capabilities. Creative Proteomics has rich experience in saponins sample handling and can analyze saponins present in a variety of plant species at cell/tissue level.

Professional data analysis skills. Our professional and technical personnel can analyze the HPLC-MS and HPLC-ESI-MS/MS data accurately, in detail and comprehensively to ensure the scientific nature and consistency of the results.

Years of plant metabolomics project management experience and mature SOP. We have professional and scientific SOP analysis procedures, which can ensure the efficient progress of customers' projects, saving time and money. We can also make one-to-one one-stop personalized service plan according to the special needs of customers.

Service Workflow

Since different tissues have different matrix properties, Creative Proteomics has developed multiple novel sample preparation techniques. Our services allow the profiling of various types of saponins, including ginsenoside, soyasaponins, etc.

Mode: MRM

Precision: pmol

Linear: R2 > 0.99

Analysis content:

• Standard curve creation
• Raw data preprocessing
• Absolute quantitation of saponins
• Differential saponins screening
• Optimal analyses

List of Detectable Saponins at Creative Proteomics

Sample Requirements

1. Fresh ginseng tissues: >1 g. If possible, samples should be frozen in liquid nitrogen right after collection, and then transferred to -80°C for storage.

2. Product samples.

At least 3 biological replicates.

Delivery

• Experimental procedure
• Parameters of HPLC and MS
• Purity analysis report
• HPLC-MS/ HPLC-ESI-MS/MS raw data files and HPLC-MS/ HPLC-ESI-MS/MS data quality checks
• Metabolites quantification data
• Custom analysis report

Mass spectrometry-based profiling of saponins enables quantitative analyses of saponins in a faster, convenient, and sensitive manner. With decades of experience in mass spectrometry services, Creative Proteomics has a proven track record supporting diverse saponins detection and quantification. We can meet your specific project requirements, from sampling to bioinformatics.