Creative Proteomics provides high performance liquid chromatography- electrospray ionization -mass spectrometry (HPLC-ESI-MS/MS) based methods for soyasaponins analysis, which provides sufficient stability, sensitivity and high resolution for identification and quantification of soyasaponins. This approach has the advantages of simple, stable, fast, accurate, specific and reproducible, which can help researchers to analyze soyasaponins effectively.
Soyasaponins is a kind of common saponin, which mainly exists in legumes. The content of soyasaponins in legumes seeds generally ranges from 0.62% to 6.16%. Soyasaponins can be divided into A group, B group, E group and DDMP group according to the structure of saponin. Soyasaponins have the general properties of saponins. Pure soyasaponins are white powder, which can irritate the mucous membrane of all parts of human body. The molecular weight of soyasaponins is about 1000. Soyasaponins have high molecular polarity, which soluble in H2O, hot water, dilute alcohol containing water, hot methanol and hot ethanol, and insoluble in organic solvents with low polarity, such as diethyl ether and benzene. Soyasaponins have a high melting point and often decompose before melting, so there is no clear melting point.
Soyasaponins belong to acidic saponins. When ammonium sulfate, lead acetate or other neutral salts are added into the aqueous solution, the precipitation will be generated, and it can be separated and extracted by this property. Soyasaponins react with benzene to produce reddish brown precipitates, and show red color in glacial acetic acid-acetyl chloride solution. These properties can be used to detect the content of soyasaponins. Creative Proteomics provides the simple, rapid, accurate, affordable and highly automated analytical method and equipment to analyse soyasaponins. High performance liquid chromatography- electrospray ionization -mass spectrometry (HPLC-ESI-MS/MS) can solve some of the problems associated with soyasaponins analysis. Improved extraction and purification steps can also increase the sensitivity of the analytical method.
Applications of Soyasaponins Analysis
- Qualitative analysis of the main types of soyasaponins
- Quantitative analysis of soyasaponins content
- Ensure safety and quality
- Provide basis for improvement
Advantages of Our Soyasaponins Analysis Service
Stable and high quality team to provide professional soyasaponins analysis services required by customers
Years of plant metabolomics project management experience and mature SOP
Professional HPLC-ESI-MS/MS data analysis skills
Our newly developed sample preprocessing method provides safety, environmental protection, rapidness, effectiveness, and recovery rates consistently exceeding 85%. Sample preprocessing at Creative Proteomics complies with the in-house standard operation procedures (SOP), ensuring maximized homogeneity between samples.
Figure 1. Soyasaponins analysis service workflow.
Detector: UV-Vis detector
Mobile phase: Acetonitrile-ammonium acetate aqueous solution
Elution time: dozens of minutes, slightly different according to different ingredients
- Standard curve creation
- Raw data preprocessing
- Absolute quantitation of soyasaponins
- Differential metabolites screening
- Optimal analyses
List of Detectable Soyasaponins at Creative Proteomics
1. Fresh ginseng tissues: >1 g. If possible, samples should be frozen in liquid nitrogen right after collection, and then transferred to -80°C for storage.
2. Product samples.
At least 3 biological replicates.
- Experimental procedure
- Parameters of HPLC-ESI-MS/MS
- Purity analysis report
- HPLC-ESI-MS/MS raw data files and HPLC-ESI-MS/MS data quality checks
- Metabolites quantification data
- Custom analysis report
HPLC-ESI-MS/MS based profiling of soyasaponins enables quantitative analyses in a faster, more convenient, and more sensitive manner. With decades of experience in HPLC-ESI-MS/MS services, Creative Proteomics has a proven track record supporting diverse soyasaponins detection and quantitation. We can meet your specific project requirements, from sample to bioinformatics.
For Research Use Only. Not for use in diagnostic procedures.