Coumarins Analysis

Overview

Coumarins are first found in the black bean. They exist in the form of glycosides in the black bean, sweet snake whipweed, wild vanilla, orchid and other plants, and have the fragrance of fresh hay and sweet bean. Coumarins are white crystalline solid, insoluble in cold water, soluble in hot water, ethanol, ether and chloroform. Coumarins can be used in the blending of soap and various detergents, as the main component of the blending of spices, and they also can be used as electroplating brightener. In addition, coumarins have a similar structure to vitamin K, which can inhibit the synthesis of blood clotting factors in the liver, and can be used as an anticoagulant. HPLC and MS are sensitive analytical technique that enables detection, identification and quantification of a variety of coumarins by measuring and characterizing their chemical structure, even at low abundance. MS instruments combine with either LC or GC to effectively separate organic molecules from complex samples.

Applications of Coumarins Analysis

• Qualitative analysis of the main types of coumarins
• Quantitative analysis of coumarins content
• Ensure safety and quality
• Provide basis for improvement

Advantages of Our Coumarins Analysis Service

Rich experience in sample handling. Coumarins are diverse and have similar structures, so sample handling and extraction are very important for identification and analysis results. We have a complete laboratory network and professional technicians to provide reliable plant targeted metabolomics service.

High cost performance. Our laboratory can meet the needs of customers who require third-party testing services. One-stop service mode can greatly save customers' time. Comprehensive project analysis solutions can also bring the highest cost performance.

Strong analytical skills. With many years of experience in HPLC and MS service, Creative Proteomics can quickly and accurately analyze the results and conduct professional analysis.

Constantly updated. We have a complete laboratory network and professional technicians to provide reliable plant targeted metabolomics service and real-time updates of industry knowledge.

Service Workflow

Since different tissues have different matrix properties, Creative Proteomics has developed multiple novel sample preparation techniques. Our services allow the profiling of various types of coumarins, including dicoumarol, warfarin, acenocoumarol etc.

Precision: pmol

Linear: R2 > 0.99

Time: dozens of minutes, slightly different according to different ingredients

Analysis content:

• Standard curve creation
• Raw data preprocessing
• Absolute quantitation of acenocoumarol
• Differential coumarins screening
• Optimal analyses

List of Detectable Coumarins at Creative Proteomics

Sample Requirements

1. Fresh tissues: >1 g. If possible, samples should be frozen in liquid nitrogen right after collection, and then transferred to -80°C for storage.

2. Product samples.

At least 3 biological replicates.

Delivery

• Experimental procedure
• Parameters of HPLC and MS
• Purity analysis report
• HPLC, LC-MS/MS and GC-MS/MS raw data files and data quality checks
• Metabolites quantification data
• Custom analysis report

High performance liquid chromatography and mass spectrometry-based profiling of coumarins enables quantitative analyses of coumarins in a faster, convenient, and sensitive manner. With decades of experience in high performance liquid chromatography and mass spectrometry services, Creative Proteomics has a proven track record supporting diverse coumarins detection and quantification. We can meet your specific project requirements, from sampling to bioinformatics.