Acenocoumarol Analysis Service

What is Acenocoumarol?

Acenocoumarol is an oral anticoagulant, a coumarin derivative, commonly used for the prevention and treatment of thromboembolic diseases such as deep vein thrombosis, pulmonary embolism, and stroke. Acenocoumarol works by inhibiting the production of vitamin K-dependent coagulation factors, thereby reducing the risk of thrombosis.

Acenocoumarol has a narrow therapeutic range, which means that the dose must be carefully monitored to achieve the desired anticoagulant effect while avoiding the risk of bleeding. This makes accurate analysis of acenocoumarol levels in biological samples essential for ensuring the safety and efficacy of treatment.

At Creative Proteomics, we offer a range of analytical services to support the development and production of acenocoumarol and other anticoagulant drugs, including the analysis of acenocoumarol levels in various biological matrices.

We use cutting-edge technologies and methodologies to ensure that our analyses are as precise and accurate as possible, and our team of experts is committed to providing fast turnaround times without compromising on the quality of our results. We also offer custom analytical method development to meet specific client needs and help ensure the safety and efficacy of their products.

Applications of Acenocoumarol Analysis

• Study of mechanism of action: Studying the effect of acenocoumarol on the coagulation cascade and the mechanism of its anticoagulant activity provides insight into the pathophysiology of thromboembolic disease and potential targets for new anticoagulant drugs.
• Investigate drug-drug interactions: acenocoumarol is metabolized by the liver and its levels may be affected by other drugs that interact with liver metabolizing enzymes. Analysis of acenocoumarol levels in the presence of other drugs may provide insight into potential drug-drug interactions.
• Assessing the toxicity of anticoagulant drugs: acenocoumarol has a narrow therapeutic window and the risk of bleeding can be severe and life-threatening. Analysis of acenocoumarol levels in preclinical studies can help determine the dose of toxicity.
• Pharmacokinetic studies: Pharmacokinetic studies are used to assess how a drug is absorbed, distributed, metabolized, and excreted by the body. Accurate analysis of acenocoumarol levels in biological samples is necessary for pharmacokinetic studies to determine the bioavailability and half-life of the drug.
• Quality Control: Accurate analysis of acenocoumarol levels ensures that the drug is manufactured at the correct concentration and that the final product is safe and effective.

Advantages of Our Acenocoumarol Analysis Service

Rich experience in sample handling. Coumarins are diverse and have similar structures, so sample handling and extraction are very important for identification and analysis results. We have a complete laboratory network and professional technicians to provide reliable targeted metabolomics service.

High cost performance. Our laboratories can provide a one-stop-shop to save you time. Comprehensive project analysis solutions also deliver the best value for money.

Strong analytical skills. With many years of experience in HPLC-MS/MS service, Creative Proteomics can quickly and accurately analyze the mass spectrometry results and conduct professional analysis.

Service Workflow

Our newly developed sample preprocessing method provides safety, environmental protection, rapidness, effectiveness, and the recovery rate is between 85% and 105%. Sample preprocessing at Creative Proteomics complies with the in-house standard operation procedures (SOP), ensuring maximized homogeneity between samples.

Figure 1. Acenocoumarol analysis service workflow.

Extracting agent: Absolute ethyl alcohol

Linear range: RSD 0.4%-1.0%; r>0.999

Time: dozens of minutes, slightly different according to different ingredients

Analysis content:

• Standard curve creation
• Raw data preprocessing
• Absolute quantitation of acenocoumarol
• Differential metabolites screening
• Optimal analysis

Sample Requirements

• Tissue ≥ 200 mg/sample
• Plasma ≥ 200 μL/sample
• Blood ≥ 200 μL/sample
• Drugs ≥ 0.2 g/sample

We also accept other samples. Our technician will communicate with you about specific sample requirements.

In order to ensure the accuracy of the samples and reduce systematic errors during sampling, it is necessary to select more than 3 materials with the same condition for each sample. The same condition refers to the same period, basically the same phenotype, and the same part.

Delivery

• Experimental procedure
• Parameters of HPLC-MS/MS
• HPLC-MS/MS raw data files and HPLC-MS/MS data quality checks
• Metabolites quantification data
• Custom analysis report

Creative Proteomics is pleased to assist you with the identification and quantitative analysis of acenocoumarol to assist in your research. If you need to analyze other compounds, please contact us.