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Glucosinolates Analysis Service

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Glucosinolates are important secondary metabolites in cruciferous vegetables. They are found in a variety of edible plants such as cabbage, Brussels sprouts, watercress, horseradish, spiny bergamot, and radish. These natural chemicals may help plants resist pests and diseases and give cruciferous vegetables their distinctive flavor.

Glucosinolates are readily hydrolyzed by the action of endogenous mustard enzymes to produce degradation products. The formation of these products is influenced by pH and certain ions and has different physiological functions. Allyl isothiocyanate is a component that produces the characteristic aromatic odor of cruciferous vegetables such as mustard and cabbage. Indole glucosinolates, such as 3-indole methyl glucosinolate, produce compounds such as indole 3-methanol and indole-3-acetonitrile by enzymatic digestion. These compounds are biologically active and can inhibit the growth of microorganisms and have deterrent effects on certain insects and herbivores. 4-Methylthiooxybutyl glucosinolate degradation product, radish sulfur, is the most powerful enzyme inducer found so far and can inactivate oncogenes.

Creative Proteomics offers metabolomics services based on LC-MS technology, which enables qualitative and quantitative analysis of glucosinolates.

Glucosinolate structure (side group R varies)Glucosinolate structure (side group R varies)

Analysis List of Glucosinolates

GlucosinolatesChemical formulaCAS number
Allylglucosinolate (Sinigrin)C10H16KNO9S23952-98-5
Sinalbin (P-hydroxybenzyl Glucosinolate)C14H19NO9S2499-26-3
Methyl GlucosinolateC15H21NO9S2499-30-9
Glucoraphanin (4-methylsulfinylbutyl Glucosinolate)C12H23NO10S321414-41-5

※ The project items are constantly being updated, not only those listed, please contact us to get more latest information and related information.

Detection Platform

  • LC-MS/MS
  • Waters ACQUITY UPLC, MS-SCIEX QTRAP 4500/5500/6500 and Waters Xevo TQ-S

  • GC-MS
  • Agilent 7890B-5977A, Thermo TRACE 1300-TSQ 9000

Technical Route of Targeted Metabolomics of Glucosinolates

Technical Route of Targeted Metabolomics of Glucosinolates

Our Advantages

  • Extensive testing items
    With rich project experience, Creative Proteomics has completed metabolic studies of hundreds of specie.
  • Personalized experimental program
    With a wealth of project analysis cases, suitable analysis solutions can be selected according to project needs to ensure accurate results.
  • Professional testing and analysis capabilities
    Strict quality control system combined with high performance liquid chromatography-mass spectrometry system, as well as professional data preprocessing and analysis capabilities, to ensure the reliability and accuracy of data.
  • Excellent high-throughput qualitative and quantitative capabilities
    The high-resolution system combined with a rich standard library can simultaneously realize the absolute quantification of a variety of endogenous metabolites.

Service Process

Service Process of Targeted Metabolomics of Glucosinolates

For Research Use Only. Not for use in diagnostic procedures.
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