Metabolomics Creative Proteomics

Glucoraphanin (4-methylsulfinylbutyl Glucosinolate) Analysis Service

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About Glucoraphanin (4-methylsulfinylbutyl Glucosinolate)

Glucoraphanin is a glucosinolate, a precursor of sulforaphane.

Glucoraphanin is found in broccoli, mustard, and some other cruciferous vegetables. And it is especially rich in young broccoli sprouts and cauliflower.

The molecular formula of Glucoraphanin is C12H23NO10S3, and its CAS number is 21414-41-5.

MS-based technical means can realize the qualitative and quantitative analysis of glucoraphanin (4-methylsulfinylbutyl glucosinolate).

Structure of glucoraphanin (4-methylsulfinylbutyl glucosinolate)Structure of glucoraphanin (4-methylsulfinylbutyl glucosinolate)

Detection Platform

  • LC-MS/MS
  • Waters ACQUITY UPLC, MS-SCIEX QTRAP 4500/5500/6500 and Waters Xevo TQ-S

  • GC-MS
  • Agilent 7890B-5977A, Thermo TRACE 1300-TSQ 9000

Technical Workflow

  • Experimental Design
  • Collect samples and prepare standards
  • Test standards and sample metabolites
  • Substance standard curve preparation
  • Raw data preprocessing
  • Absolute quantification of substances
  • Advanced analysis and customized analysis

Application Field

  • Accurate quantification of glucoraphanin (4-methylsulfinylbutyl glucosinolate)
  • Validate hypotheses proposed by non-targeted metabolomics or other omics
  • Research metabolic models for glucoraphanin (4-methylsulfinylbutyl glucosinolate)

Technical Route of Targeted Metabolomics of Glucoraphanin (4-methylsulfinylbutyl Glucosinolate)

Technical Route of Targeted Metabolomics of Glucoraphanin (4-methylsulfinylbutyl Glucosinolate)

Sample Preparation Suggestions

Sample typeSuggested sample quantity (per case)Number of repetitions (recommended)
Plant tissue≥ 400 mgPlant samples ≥ 3 cases / group
Clinical samples ≥ 30 cases / group
Animal samples ≥ 10 cases / group
Cell samples ≥ 6 cases / group
Plant seed≥ 200 mg
Blood (serum, plasma)≥ 200 μl
Urine≥ 200 μl
Animal tissue≥100 mg
Cell supernatant≥ 1ml
Bacteria or fungiWet weight of sediment ≥ 200 mg
Fermentation broth≥ 500 μl
Other special samplesPlease contact us for consultation

Feedback to Customers

Creative Proteomics will provide you with detailed technical reports, including

  • Experimental steps
  • Related mass spectrometry parameters
  • Part of the mass spectrum picture
  • Raw data
  • Quantitative results of targeted metabolites

Project Cycle

  • A standard experimental procedure takes about 1 ~ 4 weeks.

Technical Advantages

  • Mature database
  • Use mature databases (source databases and self-built libraries) for full material qualitative and reliable structure qualitative.

  • Strict QC
  • The most stringent quality control system is adopted to ensure the qualitative and quantitative results of each project.

  • Advanced platform
  • Using platforms such as Agilent 7890B-5977A, it has high sensitivity and strong qualitative ability.

  • Experienced information analysis team
  • With an experienced team provides customers with personalized biological information analysis services to ensure the accuracy and reliability of the analysis results.

For Research Use Only. Not for use in diagnostic procedures.


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