Metabolomics Creative Proteomics

Creative Proteomics Metabolomics

Sinalbin (P-hydroxybenzyl Glucosinolate) Analysis Service

Sinalbin (P-hydroxybenzyl Glucosinolate) Analysis Service

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Creative Proteomics' targeted metabolomics services target specific metabolites and pathways of interest, which can accurately monitor dynamic metabolic processes, reveal related metabolic mechanisms and verify potential metabolic biomarkers, to guide your research with reliable and accurate measurement results.

About Sinalbin (P-hydroxybenzyl Glucosinolate)

Sinalbin is a glucosinolate, found in the seeds of white mustard, Sinapis alba and many wild plant species.

Sinalbin is metabolized by myrosinase to form mustard oil (4-hydroxybenzyl isothiocyanate).

Its molecular formula is C14H19NO10S2, and its CAS number is 19253-84-0.

MS-based technical means can realize the qualitative and quantitative analysis of sinalbin (P-hydroxybenzyl glucosinolate).

Structure of sinalbin (P-hydroxybenzyl glucosinolate)Structure of sinalbin (P-hydroxybenzyl glucosinolate)

Detection Platform

  • LC-MS/MS
  • Waters ACQUITY UPLC, MS-SCIEX QTRAP 4500/5500/6500 and Waters Xevo TQ-S

  • GC-MS
  • Agilent 7890A-5975C, Agilent 7890B-5977A, Thermo TRACE 1300-TSQ 9000

Technical Advantages

  • With standardized laboratory operation specifications and advanced instrument platform, the experiment period is short and the quality is reliable.
  • It has multiple targeted instrument platforms such as Waters Quattro Premier XE triple quadrupole liquid-mass spectrometer (MRM), Agilent 7890A-5975C gas-mass spectrometer (SIM), etc.
  • With strict quality control (internal standard and external standard), and manual proofreading standard products, the standard R2>0.99
  • With a professional biological information team and large-scale hardware, it can provide customers with personalized biological information analysis services.

Application Field

  • Accurate quantification of sinalbin (P-hydroxybenzyl glucosinolate)
  • Validate hypotheses proposed by non-targeted metabolomics or other omics
  • Research metabolic models for sinalbin (P-hydroxybenzyl glucosinolate)

Technical Route of Targeted Metabolomics of Sinalbin (P-hydroxybenzyl Glucosinolate)

Technical Route of Targeted Metabolomics of Sinalbin

  • Sample preparation
  • Extract metabolites, precipitate proteins, etc.

  • Pre-test
  • Optimize the corresponding parameters, establish the chromatographic and mass spectrometry analysis conditions of the tested substance, and create the MRM analysis method.

  • Make a standard curve
  • Configure a series of different concentration standards and make a standard curve.

  • Sample detection and result analysis
  • According to the results of the sample detection, combined with the standard curve, the statistical analysis of the substance content in the sample is carried out.

Sample Preparation

Sample typeSuggested sample quantity (per case)Number of repetitions (recommended)
Plant tissue≥ 400 mg       Plant samples ≥ 3 cases / group
       Clinical samples ≥ 30 cases / group
       Animal samples ≥ 10 cases / group
       Cell samples ≥ 6 cases / group
Plant seed≥ 200 mg
Blood (serum, plasma)≥ 200 μl
Urine≥ 200 μl
Animal tissue≥100 mg
Cell107
Cell supernatant≥ 1ml
Bacteria or fungiWet weight of sediment ≥ 200 mg
Fermentation broth≥ 500 μl
Other special samplesPlease contact us for consultation

Feedback to Customers

Creative Proteomics will provide you with detailed technical reports, including

  • Experimental steps
  • Related mass spectrometry parameters
  • Part of the mass spectrum picture
  • Raw data
  • Quantitative results of targeted metabolites

Project Cycle

  • A standard experimental procedure takes about 1 ~ 4 weeks.
For Research Use Only. Not for use in diagnostic procedures.

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