Expires: Nov.15 — Dec.5, 2021
Creative Proteomics can provide a highly sensitive and easy-to-operate ultra-high performance liquid chromatography-mass spectrometry (UPLC-MS/MS) for the determination of isoflavone content, which can be applied to the rapid and efficient determination of isoflavone samples. This method can meet the needs of high-throughput determination of isoflavones, and has the characteristics of simplified operating procedures and high accuracy.
Natural isoflavone compounds are widely found in the plant kingdom and are the effective ingredients of many herbal medicines. They have the effects of estrogen-like, anti-tumor, prevention and treatment of cardiovascular diseases, bone metabolism, neuroprotection and improvement of cognition. Some natural isoflavones have strong antioxidant, anti-inflammatory and antibacterial effects. Isoflavones are widely used in medicine for cardiovascular, tumor, endocrine, etc., as well as health functional foods for freckle beauty and anti-aging. Due to its higher resolution and sensitivity, UPLC-MS/MS is increasingly used in drug analysis, which is beneficial to the determination of multi-index components and provides qualitative and quantitative means for the selection of rational quality control indicators. UPLC-MS/MS has the advantages of simple operation and high accuracy, which can be used for the quality control of isoflavones and provide guidance information for the development and utilization of natural isoflavones.
Figure 1. Differentially accumulated isoflavonoids in GmMYB176-Si and GmMYB176-OE hairy roots compared to controls. (Anguraj 2019)
No derivatization is required for sample processing in our operating technical process, which can reduce human error of the sample to be tested. UPLC-MS/MS can achieve high-efficiency separation of samples and a good recovery rate. The cycle of analyzing the samples to be tested is short, which can meet the needs of high-throughput determination of isoflavone samples.
Figure 2. Isoflavonoids analysis service workflow.
Detection method: UPLC-MS/MS
Mobile phase: acetonitrile-water (containing 0.1% formic acid)
Elution method: gradient elution
Volume flow: 0.25ml/min
Average sample recovery rate: 97.53%-104.30%
Injection volume: 2ul
Detection wavelength: 276nm
Reaction mode: positive ion multiple reaction monitoring (MRM) mode
1. Tissues such as leaves, roots, stems, flowers, fruit pulp, peel tissue, etc., larger leaves need to be quickly cut into pieces with scissors, and the separated samples are quickly washed with sterilized water, and the absorbent paper is immediately put into the liquid after absorbing the excess water. Quickly freeze in nitrogen for more than 2 minutes, and then transfer to -80°C for storage to avoid repeated freezing and thawing.
2. For each sample, take no less than 3 g for fresh samples, no less than 1 g for dry samples. At least three biological replicates in each group, prepare a backup. The measured sample will not be returned, please keep a backup.
UPLC-MS/MS can realize the high-efficiency determination of isoflavone compounds, meet the actual determination of multiple types of isoflavone samples, and has a short analysis cycle and simplified operation process. Creative Proteomics has a first-class UPLC-MS/MS measurement platform and experienced analysis engineers, which can meet your needs for customized one-stop service.
Expires: Nov.15 — Dec.5, 2021