Metabolomics Creative Proteomics

Creative Proteomics Metabolomics

Nicotinamide Adenine Dinucleotide Analysis Service

Nicotinamide Adenine Dinucleotide Analysis Service

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About Nicotinamide Adenine Dinucleotide

Nicotinamide adenine dinucleotide (NADH) is a chemical substance that is the reduced form of nicotinamide adenine dinucleotide, reduced coenzyme I.

Because NADH is mainly involved in material and energy metabolism in cells, it is produced in the citric acid cycle during glycolysis and cellular respiration, and it acts as a carrier and electron donor for biological hydrogen. Through the process of oxidative phosphorylation on the inner mitochondrial membrane, energy is transferred for ATP synthesis. So NADH is also called mitochondrial.

NADH plays an important role in maintaining cell growth, differentiation, energy metabolism, and cell protection.

Its chemical formula is C21H27N7O14P2, and CAS number are 53-84-9, 53-59-8 (phosphate), 58-68-4 (NADH).

MS-based technical means can realize the qualitative and quantitative analysis of nicotinamide adenine dinucleotide.

Molecular structure of nicotinamide adenine dinucleotideMolecular structure of nicotinamide adenine dinucleotide

Technical Route of Targeted Metabolomics of Nicotinamide Adenine Dinucleotide

Molecular structure of nicotinamide adenine dinucleotide

Sample Requirements

Sample type
Plants, yeasts, microorganisms, etc.
     Serum, plasma, urine, bile, bile acid, etc.
     Cells, liver, brain tissue and other animal tissues and feces, etc.
     There is no restriction on plant varieties. For varieties with special requirements or rare varieties, please contact our staff for more information.
Sample demand
Plant root / stem / leaf / fruit / seed > 200 mg fresh weight, freeze-dried sample > 0.5 g
     Cells / microorganisms / cell supernatant / culture medium supernatant: the number of cells or bacteria > 107, supernatant > 2 ml
     Serum / plasma / urine / ruminal fluid / cerebrospinal fluid / amniotic fluid and other homogeneous liquid samples > 200 µl.
     (Hemolysis should be avoided. Cerebrospinal fluid can be as low as 100 µl.)
     Animal tissue / feces related samples > 200 mg / sample
     For other sample types, please consult technical support or sales.
Sample mixed and repeat
In order to ensure the accuracy of the samples and reduce systematic errors during sampling, it is necessary to select more than 3 materials with the same condition for each sample.
     ※ The same condition refers to the same period, basically the same phenotype, and the same part.
     It is recommended that prepare more than 6 biological replicates.
Storage and transportation
Quick-frozen preservation with liquid nitrogen can minimize the leakage time of plant samples at room temperature, and store it at -80°C.
     Dry ice transportation (about 3~4 kg dry ice is consumed every day, please use sufficient dry ice for transportation).

Feedback to Customers

Creative Proteomics will provide you with detailed technical reports, including

  • Experimental steps
  • Related mass spectrometry parameters
  • Part of the mass spectrum picture
  • Raw data
  • Metabolic molecular identification results

Creative Proteomics offers several approaches to metabolomics studies, delivers precise and detailed data and analysis report. We can also customize the methods or establish new methods together with our collaborators, so they are fit-for-purpose and meet your specific needs. If you have any questions or specific requirements, please feel free to contact us.

References

  1. Riera-Borrull M, Rodríguez-Gallego E, Hernández-Aguilera A, et al. Exploring the process of energy generation in pathophysiology by targeted metabolomics: performance of a simple and quantitative method. Journal of the American Society for Mass Spectrometry, 2015, 27(1): 168-177.
  2. Kim M J, Lee M Y, Shon J C, et al. Untargeted and targeted metabolomics analyses of blackberries – Understanding postharvest red drupelet disorder. Food Chemistry, 2019, 300:125169.
  3. Wang X, Zhao X, Zhao J, et al. Serum metabolite signatures of epithelial ovarian cancer based on targeted metabolomics. Clinica Chimica Acta, 2021, 518: 59-69.
For Research Use Only. Not for use in diagnostic procedures.
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