What is Nicotinamide Adenine Dinucleotide?
Nicotinamide Adenine Dinucleotide (NAD) is a coenzyme composed of two nucleotides: nicotinamide and adenine dinucleotide. It exists in two forms, NAD+ and NADH, which can convert between each other in redox reactions. NAD+ is involved in catabolic reactions as the oxidized form of the coenzyme, while NADH participates in anabolic reactions as the reduced form.
NAD is crucial for energy metabolism, specifically in the process of cellular respiration. It helps oxidize glucose and other fuels to produce ATP, the primary energy currency of cells. NAD is also involved in the biosynthesis of fats, cholesterol, and steroids, and plays a role in DNA repair, gene expression, and circadian rhythm regulation. Overall, NAD is an essential molecule in various biological processes due to its multifaceted functions.
Molecular structure of nicotinamide adenine dinucleotide
What is Nicotinamide Adenine Dinucleotide used for?
NAD, a pivotal coenzyme, performs an indispensable function in cellular processes. The criticality of NAD has spurred its applications in a plethora of domains, especially in the realm of NAD analysis. This investigative practice encompasses the measurement of NAD levels in cells, tissues, or biological fluids, presenting valuable insights into cellular metabolism. This analytical tactic, in turn, can be utilized to diagnose and monitor various diseases, including but not limited to cancer, diabetes, and neurological disorders.
The broad spectrum of NAD analysis finds extensive applications in diverse areas, such as drug discovery, cancer research, and personalized medicine. In drug discovery, the tactical implementation of NAD analysis can be employed to screen and identify potential drugs that can influence NAD metabolism. The understanding of metabolic changes caused by NAD offers an avenue for developing drugs that can modulate its activity. In a similar vein, the application of NAD analysis in cancer research can be used to investigate the metabolic changes in cancer cells, a vital step in developing targeted therapies. This innovative approach enables researchers to identify novel targets and pathways for developing cancer treatments.
NAD Analysis in Creative Proteomics
At Creative Proteomics, we are dedicated to providing top-notch NAD analysis services to our clients. Our team of experts boasts extensive experience in the field and is committed to utilizing state-of-the-art techniques, such as HPLC-MS, to ensure the utmost accuracy and precision in our results. Our NAD analysis services include:
- NAD/NADH measurement
- NADP/NADPH measurement
- NAD kinase activity assay
- NAD glycohydrolase activity assay
We provide NAD analysis services for various sample types, including cells, tissues, and biological fluids. Our services can be customized to meet your specific needs and requirements.
Technical Route of Targeted Metabolomics of Nicotinamide Adenine Dinucleotide
Sample Requirements for Nicotinamide Adenine Dinucleotide Assay
|Sample Types||Sample Requirements||Analysis Techniques|
|Whole Blood||Cellular components need to be lysed||HPLC, MS|
|Plasma/Serum||Anticoagulant for plasma samples, no anticoagulant for serum samples||HPLC, MS|
|Urine||Collection of urine sample||LC-MS/MS|
|Cerebrospinal Fluid (CSF)||Lumbar puncture procedure||HPLC, MS|
|Tissue Samples (Animal)||Tissue homogenization, extraction with organic solvents||HPLC, LC-MS/MS|
|Plant Samples||Plant tissue (leaves, stems, roots, etc.)||HPLC, LC-MS/MS|
Please note that this table provides a brief overview, and specific protocols may vary depending on the study design and the sensitivity required for NAD+ analysis in each bodily fluid sample.
Feedback to Customers
Creative Proteomics will provide you with detailed technical reports, including
- Experimental steps
- Related mass spectrometry parameters
- Part of the mass spectrum picture
- Raw data
- Metabolic molecular identification results
Creative Proteomics offers several approaches to metabolomics studies, delivers precise and detailed data and analysis report. We can also customize the methods or establish new methods together with our collaborators, so they are fit-for-purpose and meet your specific needs. If you have any questions or specific requirements, please feel free to contact us.
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- Kim M J, Lee M Y, Shon J C, et al. Untargeted and targeted metabolomics analyses of blackberries – Understanding postharvest red drupelet disorder. Food Chemistry, 2019, 300:125169.
- Wang X, Zhao X, Zhao J, et al. Serum metabolite signatures of epithelial ovarian cancer based on targeted metabolomics. Clinica Chimica Acta, 2021, 518: 59-69.
For Research Use Only. Not for use in diagnostic procedures.