Metabolomics Creative Proteomics

Creative Proteomics Metabolomics

Citrate Cycle Metabolism Service

Citrate Cycle Metabolism Service

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Creative Proteomics, as a leading biotechnology company, provides reliable, rapid and cost-effective citrate cycle (TCA cycle) analysis service in animal. We leverage our experience, expertise, and technologies to accelerate your scientific research of TCA cycle metabolism analysis in animal.

Overview

TCA cycle is the central metabolic cycle of the carbohydrate metabolism and all metabolic pathways. It begins when the pyruvate formed in the cytoplasm of the cell during glycolysis is transferred to the mitochondria, in which most of the energy inherent in glucose is extracted (Figure 1). In the mitochondria, pyruvate is converted to acetyl CoA by the enzyme pyruvate carboxlase. Oxidation of acetyl-CoA by TCA cycle accounts for 2/3rd of total oxygen consumption and ATP production1. TCA cycle plays an important role in many biosynthetic pathways like gluconeogenesis and transamination. deamination and lipogenesis. Therefore, aberrant TCA cycle function contributes to various diseases like cancer2. As a high-throughput tool, TCA cycle metabolites analysis provides a powerful way to diagnosis and gain new insights in disease mechanism or treatment. With integrated array of separation, characterization, identification and quantification experience, Creative Proteomics provides reliable, rapid and cost-effective TCA cycle metabolites analysis service for your various scientific purposes.

The overview of TCA cycle.Figure 1. The overview of TCA cycle.

The Main Applications of TCA Cycle Metabolites Service

  • Biomarker discovery
  • Disease diagnostics
  • Drug discovery
  • Food safety
  • Metabolic disease research

Advantages of Our TCA Cycle Metabolites Service

Rich experience in different types of animal sample preparation

State of art facilities

Professional experiment design and data analysis

Competitive price

Short turnaround time

High accuracy, specificity, and sensitivity

Service Workflow

The TCA cycle metabolites service provided by Creative Proteomics is based on our cutting-edge chromatographic separation and mass spectrometry platforms. The experimental workflow involves 4 steps: sample collection, metabolites extraction, MS data analysis and bioinformatics analysis (Figure 1). Our service will be tailored to specific samples and needs for optimal results.

The overall workflow of TCA cycle metabolism service.Figure 2. The overall workflow of TCA cycle metabolism service.

List of Detectable TCA Cycle Metabolites at Creative Proteomics

Citric acidIsocitric acidα-ketoglutaric acid
Succinyl CoASuccinateFumarate
MalateOxaloacetate 

Sample Requirements

We can analyze any biological materials including but not limited to cells and solid tissues from humans and animals, such as mice, rats, rabbits. If you need transport your samples to us, please follow the below requirements for different types of sample:

  • Blood/plasma: 500ul/sample
  • Urine: 1ml/sample
  • Tissue: 200mg/sample
  • Cells: 1x107/sample
  • Feces: 500mg/sample
    Shipment condition: dry ice

Report Delivery

  • Experimental procedures
  • Instrument parameters of liquid chromatography and mass spectrometry
  • MS raw data files and the summary of mass spectrometry data quality
  • The identification/quantification of lipidic molecules data
  • Bioinformatics analysis report (PCA, KEGG, etc.)

Equipped with advanced mass spectrometry for the determination of TCA metabolites, powerful bioinformatic analysis software and a panel of experienced technicians and scientists, we provide customer-tailored TCA cycle metabolites analysis service with rapid experimental procedures and easy to read report, to advance your scientific research.

References

  1. Muhammad Akram. Citric acid cycle and role of its intermediates in metabolism. Cell Biochem Biophys. 2014, 68:475-478.
  2. Nicole M Anderson, Patrick Mucka, Joseph G Kern and Hui Feng. The emerging role and targetability of the TCA cycle in cancer metabolism. Protein Cell. 2018 ,9:216-237.
For Research Use Only. Not for use in diagnostic procedures.

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