Glycolysis is a common metabolic pathway in almost all cells. It occurs in the cytoplasm of the cell. Glycolysis consists of ten enzyme-catalyzed reactions whose main purpose is to convert glucose into pyruvate and produce energy in the form of ATP. For cells without mitochondria, like erythrocytes, glycolysis is the only source of energy for ATP. For cells containing mitochondria, glycolysis is the preparatory pathway for the complete oxidation of glucose to produce large amounts of ATP. All cells in the body use glycolysis to produce energy. Some of the intermediates in the glycolytic cycle steps may also have direct biological roles, such as dihydroxyacetone phosphate, which is used in lipid synthesis. By monitoring metabolite levels at key steps of the glycolytic pathway, we can gain useful insights into the overall energy homeostasis of biological systems. Defective glycolysis can lead to various diseases, such as cancer.
To explore in depth the relevance of glycolytic metabolic pathways and related metabolites to the pathophysiology of various diseases affecting human health, Creative Proteomics offers an integrated and comprehensive service for the analysis of glycolytic pathway key point substances and other metabolites in biological samples.
The Main Applications of Glycolysis Metabolism Service
Biomarker discovery
Disease diagnostics
Drug discovery
Food safety
Metabolic disease research
Figure 1. Glycolysis: the glucose oxidative pathway at cellular levels.
Advantages of Our Glycolysis Metabolism Service
Rich experience in different types of animal sample preparation
State of art facilities
Professional experiment design and data analysis
Competitive price
Short turnaround time
High accuracy, specificity, and sensitivity
Service Workflow
The glycolysis metabolism service provided by Creative Proteomics is based on our cutting-edge chromatographic separation and mass spectrometry platforms. The experimental workflow involves 4 steps: sample collection, metabolites extraction, MS data analysis and bioinformatics analysis (Figure 2). Our service will be tailored to specific samples and needs for optimal results.
Figure 2. The overall workflow of glycolysis metabolism service.
List of Detectable Glycolysis Metabolites at Creative Proteomics
Sample Requirements
We can analyze any biological materials including but not limited to cells and solid tissues from animals. If you need transport your samples to us, please follow the below requirements for different types of sample:
- Blood/plasma: 500ul/sample
- Urine: 1ml/sample
- Tissue: 200mg/sample
- Cells: 1x107/sample
- Feces: 500mg/sample
Shipment condition: dry ice
Report Delivery
- Experimental procedures
- Instrument parameters of liquid chromatography and mass spectrometry
- MS raw data files and the summary of mass spectrometry data quality
- Bioinformatics analysis report (PCA, KEGG, etc.)
Creative Proteomics can provide glycolytic pathway and glycolytic metabolite analysis. If you would like to analyze other metabolites or have questions, please contact us.
References
- Arren Bar-Even, Avi Flamholz, Elad Noor and Ron Milo.Rethinking glycolysis: on the biochemical logic of metabolic pathways. Nat Chem Biol. 2012,8:509-517.
- Robert A Gatenby, Robert J Gillies. Glycolysis in cancer: a potential target for therapy. Int J Biochem Cell Biol. 2007,39:1358-1366.
For Research Use Only. Not for use in diagnostic procedures.
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