Glyceraldehyde 3-phosphate Analysis Service

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Glyceraldehyde 3-phosphate (3-phosphoglyceraldehyde) is an important intermediate in the glycogen or starch enzymatic process in living organisms. It is produced in living organisms by fructose-1,6-bisphosphate catalyzed by aldolase and is involved in other subsequent glycolytic processes. Glyceraldehyde 3-phosphate plays an important role in the conversion from sugar to bioenergy and in the interconversion of various monosaccharides in the liver. Creative Proteomics provides targeted metabolites and metabolic pathway analysis to reveal relevant metabolic mechanisms and validate potential metabolic biomarkers, guiding your research with reliable and accurate measurements.

Molecular structure of glyceraldehyde 3-phosphate

Technical Route of Targeted Metabolomics of Glyceraldehyde 3-phosphate

Sample Requirements

Sample type

Plants, yeasts, microorganisms, etc.
Serum, plasma, urine, bile, bile acid, etc.
Cells, liver, brain tissue and other animal tissues and feces, etc.
There is no restriction on plant varieties. For varieties with special requirements or rare varieties, please contact our staff for more information.

Sample demand

Plant root / stem / leaf / fruit / seed > 200 mg fresh weight, freeze-dried sample > 0.5 g
Cells / microorganisms / cell supernatant / culture medium supernatant: the number of cells or bacteria > 10⁷, supernatant > 2 ml
Serum / plasma / urine / ruminal fluid / cerebrospinal fluid / amniotic fluid and other homogeneous liquid samples > 200 µl.
(Hemolysis should be avoided. Cerebrospinal fluid can be as low as 100 µl.)
Animal tissue / feces related samples > 200mg / sample
For other sample types, please consult technical support or sales.

Sample mixed and repeat

In order to ensure the accuracy of the samples and reduce systematic errors during sampling, it is necessary to select more than 3 materials with the same condition for each sample.
※ The same condition refers to the same period, basically the same phenotype, and the same part.
It is recommended that prepare more than 6 biological replicates.

Storage and transportation

Quick-frozen preservation with liquid nitrogen can minimize the leakage time of plant samples at room temperature, and store it at -80℃.
Dry ice transportation (about 3~4 kg dry ice is consumed every day, please use sufficient dry ice for transportation).

Feedback to Customers

Creative Proteomics will provide you with detailed technical reports, including

Experimental steps
Related mass spectrometry parameters
Part of the mass spectrum picture
Raw data
Metabolic molecular identification results

Creative Proteomics offers several approaches to metabolomics studies, delivers precise and detailed data and analysis report. We can also customize the methods or establish new methods together with our collaborators, so they are fit-for-purpose and meet your specific needs. If you have any questions or specific requirements, please feel free to contact us.

References

Kim M J, Lee M Y, Shon J C, et al. Untargeted and targeted metabolomics analyses of blackberries – Understanding postharvest red drupelet disorder. Food Chemistry, 2019, 300:125169.
Pawlak M, Klupczynska A, Kokot Z J, et al. Extending Metabolomic Studies of Apis mellifera Venom: LC-MS-Based Targeted Analysis of Organic Acids. Toxins, 2019, 12(1):14.
Wang X, Zhao X, Zhao J, et al. Serum metabolite signatures of epithelial ovarian cancer based on targeted metabolomics. Clinica Chimica Acta, 2021, 518: 59-69.

For Research Use Only. Not for use in diagnostic procedures.

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