Nitrogen Metabolism Service

The main purpose of nitrogen metabolism is to regulate nitrogen acquisition and utilization during multiple metabolic procedures in cells. In our body, nitrogen is a very important key element. Therefore, nitrogen metabolism provides important intermediates for synthesis of other cellular components including proteins and nucleic acids. Among these intermediates, ammonia has important roles in connecting glutamate and glutamine synthesis. Amino and amide groups from these 2 substances are freely transferred to other carbon skeletons by transamination and transamination reactions. All in all, as an important form in energy metabolism, nitrogen metabolism plays important roles in a wide range of physiological conditions. Thus, its dysfunction is closely associated with various diseases like Carbamoyl phosphate synthetase I deficiency and Congenital systemic glutamine deficiency. In Creative Proteomics, we provide reliable, quick and cost-effective nitrogen metabolism analysis service to speed up your research aims ranging from diseases diagnosis to gaining new insights in disease mechanism or treatment.

The Main Applications of Nitrogen Metabolism Service

Identifying novel biomarkers of diseases

Improving diagnose diseases

Accelerating development of novel drugs

Gaining new mechanism of diseases

Advantages of Our Nitrogen Metabolism Service

Ample experience in a variety of animal sample preparation

State of art facilities

Professional standard operation procedure

Low cost

Short experimental time

High accuracy, specificity, and sensitivity

Service Workflow

Creative Proteomics offers nitrogen metabolism service in a reliable and effective manner, based on our cutting-edge high-performance liquid chromatography- mass spectrometry (HPLC-MS) platforms. The experimental procedures contain four main procedures: sample collection, metabolites extraction, HPLC-MS data analysis and bioinformatics analysis (Figure 1). Our service will be tailored to specific samples and needs for optimal results.

Figure 1. The overall workflow of nitrogen metabolism service.

The Potential Detectable Nitrogen Metabolism Related Metabolites at Creative Proteomics

Ammonia	Amino acids	Hydrazine	Formonide
Formic acid	Carbamate	Hydroxylamine	Cyanate
Carbamoyl phosphate	L-Glutamine	Nitrite	L-Glutamate

Sample Requirements

We can analyze a wide range of biological materials including but not limited to cells and solid tissues from humans and animals, such as mice, rats, rabbits. If you need transport your samples to us, please follow the below requirements for different kinds of sample:

• Blood/plasma: 500ul/sample
• Urine: 1ml/sample
• Tissue: 200mg/sample
• Cells: 1x10⁷/sample
• Feces: 500mg/sample
• Shipment condition: dry ice

Report Delivery

• Experimental protocols
• Instrumental factors of HPLC
• The raw data files of HPLC and the summary of HPLC data quality
• Bioinformatics analysis report

Based on advanced HPLC platforms for the determination of nitrogen metabolism, professional bioinformatic analysis software and skilled technicians and scientists, Creative Proteomics provides customer-tailored nitrogen metabolism analysis service with rapid experimental procedures and easy to read report, to accelerate your scientific research.

References

1. Tomé D, Bos C. Dietary protein and nitrogen utilization. J Nutr. 2000.130:1868S-1873S.
2. Rose Christopher, Jalan Rajiv. Congenital glutamine deficiency with glutamine synthetase mutations. N Engl J Med.2006. 354:1093-1094.
3. Kurokawa Keiji, Yorifuji Tohru, Kawai Masahiko, et al. Molecular and clinical analyses of Japanese patients with carbamoylphosphate synthetase 1 (CPS1) deficiency. J Hum Genet. 2007.52:349-354.