Sulfur Metabolism Service

Sulfur is an essential element in mammalian and occurs in various oxidation states ranging from sulfate to sulfide. Sulfate reduction can occur in both the energy producing dissimilatory pathway and the energy consuming assimilatory pathway. In dissimilatory pathway sulfate (or sulfur) is the terminal electron acceptor of the respiratory chain producing large quantities of inorganic sulfide. In contrast, the assimilatory pathway produces reduced sulfur compounds for the biosynthesis of S-containing amino acids and does not lead to direct excretion of sulfide. What is important, both pathways begin from the activation of sulfate by reaction with ATP to form adenylyl sulfate (APS). Therefore, abnormal sulfur metabolism could induce ATP depletion causing the energy metabolism disorder. It would not be surprising that sulfur metabolism play important roles in various human diseases like sulfite oxidase deficiency and chondrodysplasia with joint dislocations. In Creative Proteomics, we provide reliable, fast and cost-effective sulfur metabolism analysis service to speed up your research aims ranging from diseases diagnosis to gaining new insights in disease mechanism or treatment.

The Main Applications of Sulfur Metabolism Service

Identifying novel biomarkers of diseases

Improving diagnose diseases

Accelerating development of novel drugs

Gaining new mechanism of diseases

Advantages of Our Sulfur Metabolism Service

Ample experience in a variety of animal sample preparation

State of art facilities

Professional standard operation procedure

Low cost

Short experimental time

High accuracy, specificity, and sensitivity

Service Workflow

Creative Proteomics offers sulfur metabolism service in a reliable and effective manner, based on our cutting-edge high-performance liquid chromatography- mass spectrometry (HPLC-MS) platforms. The experimental procedures contain four main procedures: sample collection, metabolites extraction, HPLC-MS data analysis and bioinformatics analysis (Figure 1). Our service will be tailored to specific samples and needs for optimal results.

Figure 1. The overall workflow of sulfur metabolism service.

The Potential Detectable Sulfur Metabolism Related Metabolites at Creative Proteomics

Sulfate	Sulfite	Sulfide	L-Cystenine
Methanesulfonic acid	Glutathione hydrodisulfide	L-Homocysteine	Sulfonylbismethane
Methylmercaptan	Dimethyl sulfide	3-Methylthiopropionate	Adenylyl sulfate

Sample Requirements

We can analyze a wide range of biological materials including but not limited to cells and solid tissues from humans and animals, such as mice, rats, rabbits. If you need transport your samples to us, please follow the below requirements for different type of sample:

• Blood/plasma: 500ul/sample
• Urine: 1ml/sample
• Tissue: 200mg/sample
• Cells: 1x10⁷/sample
• Feces: 500mg/sample
• Shipment condition: dry ice

Report Delivery

• Experimental protocols
• Instrumental parameters of HPLC
• Bioinformatics analysis report

Based on advanced HPLC platforms for the determination of sulfur metabolism, professional bioinformatic analysis software and skilled technicians and scientists, Creative Proteomics provides customer-tailored nitrogen metabolism analysis service with rapid experimental procedures and easy to read report, to accelerate your scientific research.

References

1. Nizon Mathilde, Alanay Yasemin, Tuysuz Beyhan, et al. IMPAD1 mutations in two Catel-Manzke like patients. Am J Med Genet A. 2012.158:2183-2187.
2. Tan Wen-Hann, Eichler Florian S, Hoda Sadaf, et al. Isolated sulfite oxidase deficiency: a case report with a novel mutation and review of the literature. Pediatrics. 2005.116:757-766.