Flavonols Analysis

Overview

Flavonols refer to compounds containing 2-phenyl-3-hydroxyl (or oxygen-substituted) benzo-pyranone (2-phenyl-3-hydroxyl monochromone), which are the most abundant and widely distributed flavonoid compounds. One of the simplest flavonols is 7-monohydroxy flavonols. The only molecule that contains chlorine-substituted flavonols is chlorflavanin, which has antifungal activity. The most oxygen-substituted flavonols is digicitrin. Quercetin is the most widely distributed and common flavonols in the plant kingdom. Rutin is the most common flavonols glycoside compound. 6-C-β-D-glucosylquercetin is one of the few flavonol glycosides found. Flavonols can modulate the body's responsiveness to a number of compounds, including allergens, viruses, and carcinogens. Flavonols have anti-allergic, anti-inflammatory and anti-cancer properties, and can also improve the immune system function of the body. Mass spectrometry is a sensitive analytical technique that enables detection, identification and quantification of a variety of flavonols by measuring their mass and characterizing their chemical structure, even at low abundance. MS instruments combine with high performance liquid chromatography (HPLC) to effectively separate organic molecules from complex samples.

Applications of Flavonols Analysis

• Qualitative analysis of the main types of flavonols
• Quantitative analysis of flavonols content
• Ensure safety and quality
• Provide basis for improvement

Advantages of Our Flavonols Analysis Service

Excellent flavonols qualitative and quantitative capabilities. Creative Proteomics has rich experience in flavonols sample handling and can analyze flavonols present in a variety of plant species at cell/tissue level.

Professional data analysis skills. Our professional and technical personnel can analyze the HPLC-MS/MS data accurately, in detail and comprehensively to ensure the scientific nature and consistency of the results.

Years of plant metabolomics project management experience and mature SOP. We have professional and scientific SOP analysis procedures, which can ensure the efficient progress of customers' projects, saving time and money. We can also make one-to-one one-stop personalized service plan according to the special needs of customers.

Service Workflow

Since different tissues have different matrix properties, Creative Proteomics has developed multiple novel sample preparation techniques. Our services allow the profiling of various types of flavonols, including rutin, quercetin, etc.

Mode: MRM

Precision: pmol

Linear: R2 > 0.99

Analysis content:

• Standard curve creation
• Raw data preprocessing
• Absolute quantification of flavonols
• Differential metabolites screening
• Optimal analyses

List of Detectable Flavonols at Creative Proteomics

Sample Requirements

1. Fresh tissues: >1 g. If possible, samples should be frozen in liquid nitrogen right after collection, and then transferred to -80°C for storage.

2. Product samples.

At least 3 biological replicates.

Deliverables

• Experimental procedure
• Parameters of HPLC and MS
• Purity analysis report
• HPLC-MS/MS raw data files and HPLC-MS/MS data quality checks
• Metabolites quantification data
• Custom analysis report

Mass spectrometry-based profiling of flavonols enables quantitative analyses of flavonols in a faster, convenient, and sensitive manner. With decades of experience in mass spectrometry services, Creative Proteomics has a proven track record supporting diverse flavonols detection and quantification. We can meet your specific project requirements, from sampling to bioinformatics.