What is Urolithin A?
Urolithin A is a bioactive compound formed through the gut microbiota-mediated metabolism of ellagic acid and ellagitannins, commonly found in various fruits such as pomegranates, strawberries, and raspberries. This metabolite has been associated with numerous health benefits, including anti-inflammatory, antioxidant, and anti-cancer properties. Understanding the metabolic fate of Urolithin A is crucial for harnessing its potential therapeutic effects.
Analyzing Urolithin A provides insights into its metabolism, bioavailability, and distribution within biological systems. This information aids researchers and healthcare professionals in designing targeted interventions for various health conditions. Creative Proteomics, a pioneer in advanced metabolomics analysis, offers a comprehensive suite of services for Urolithin A metabolic profiling. Our cutting-edge technologies enable the identification and quantification of Urolithin A and its metabolites, unveiling the intricate metabolic pathways involved.
Key Aspects of Our Urolithin A Metabolomics Analysis
Precise Metabolic Pathway Elucidation: Employing state-of-the-art metabolomics technologies, we unravel the intricate metabolic pathways leading to Urolithin A production. Gain a comprehensive understanding of how gut microbiota convert ellagitannins into this bioactive compound.
Customized Solutions for Research: Our services are precisely tailored to align with your specific research goals. Investigate Urolithin A's potential role in oxidative stress modulation, inflammation attenuation, and implications for health.
Advancing Biopharmaceutical Innovations: Explore the therapeutic potential of Urolithin A in biopharmaceutical applications. Leverage our analyses to drive the development of innovative interventions targeting conditions related to oxidative stress and inflammatory diseases.
Personalized Nutritional Insights: Gain insights into individual variability in Urolithin A metabolism. Utilize this knowledge to create personalized dietary recommendations, contributing to comprehensive health management.
Urolithin A Analysis Project in Creative Proteomics
Metabolite Identification: Utilizing state-of-the-art mass spectrometry-based techniques, we identify Urolithin A and its metabolites with high precision. Our advanced instrumentation, such as [Instrument Model Name], ensures accurate and reliable results.
Metabolite Quantification: Quantitative analysis of Urolithin A and related metabolites is essential for understanding their concentrations in different biological samples.
Metabolic Pathway Mapping: Our experts perform metabolic pathway mapping to elucidate the sequence of reactions involved in Urolithin A metabolism, shedding light on potential biomarkers and therapeutic targets.
Bioinformatics Analysis: Creative Proteomics employs sophisticated bioinformatics tools to interpret complex metabolomic data, facilitating comprehensive insights into Urolithin A metabolism.
Urolithin A Assay Platforms
Liquid Chromatography-Mass Spectrometry (LC-MS):
This powerful combination enables high-resolution and accurate mass measurements, facilitating the precise identification and quantification of Urolithin A and its metabolites.
- Instrumentation: Thermo Scientific Q Exactive™ Plus Hybrid Quadrupole-Orbitrap™ Mass Spectrometer
- LC System: Agilent 1290 Infinity II LC System
Gas Chromatography-Mass Spectrometry (GC-MS):
The GC-MS platform excels in analyzing volatile and thermally stable Urolithin A metabolites, providing valuable insights into its metabolic pathways.
- Instrumentation: Agilent 7890B Gas Chromatograph coupled with Agilent 5977A Mass Selective Detector (MSD)
- GC System: Agilent 7890B Gas Chromatograph
Tandem Mass Spectrometry (MS/MS):
This cutting-edge triple quadrupole instrument offers exceptional sensitivity and selectivity for targeted Urolithin A metabolite analysis, allowing for detailed structural elucidation.
- Instrumentation: AB SCIEX QTRAP® 6500+ LC-MS/MS System
High-Resolution Mass Spectrometry:
This high-resolution platform enables accurate mass measurements and advanced data acquisition strategies, making it ideal for comprehensive Urolithin A metabolomics studies.
- Instrumentation: Waters SYNAPT G2-Si High Definition Mass Spectrometry (HDMS) System
Metabolite Profiling Software:
We utilize industry-standard software such as Xcalibur™ and Analyst® to process, analyze, and interpret the complex metabolomics data generated by our mass spectrometry platforms.
![LC−MS−MS analysis of fecal extracts from a donor 29 h after walnut ingestion[1]. (A) UV chromatogram at 360 nm; (B) extracted ion chromatogram (EIC) at m/z+ 229 showing the metabolite urolithin A.](upload/image/urolithin-a-analysis-service-1.jpg)
LC−MS−MS analysis of fecal extracts from a donor 29 h after walnut ingestion[1]. (A) UV chromatogram at 360 nm; (B) extracted ion chromatogram (EIC) at m/z+ 229 showing the metabolite urolithin A.
Sample Requirements for Urolithin A Assay
| Sample Type | Minimum Sample Quantity | Preferred Sample Quantity | Notes |
|---|
| Plasma / Serum | 100 μL | 200-500 μL | Anticoagulant-free tubes are recommended. |
| Urine | 1 mL | 2-5 mL | Collect in a sterile container. |
| Tissue (e.g., muscle) | 20 mg | 50-100 mg | Snap-freeze in liquid nitrogen if possible. |
| Cell Culture Supernatant | 500 μL | 1-2 mL | Centrifuge to remove cellular debris. |
| Feces | 100 mg | 200-500 mg | Collect in a sterile container. |
Applications of Urolithin A Analysis
Nutrition and Dietary Studies
Urolithin A is a bioactive compound formed through the gut metabolism of ellagic acid, commonly found in select fruits and nuts. Our analysis services can help you understand how ellagic acid-rich foods such as pomegranates, berries, and nuts contribute to the production of Urolithin A in the gut. This sheds light on the potential health benefits of these foods and their impact on gut microbiota.
Gut Microbiota Research
Studying Urolithin A contributes to investigating the role of gut microbiota in metabolizing lipid compounds. Analyzing Urolithin A levels provides insights into the relationship between gut microbiota composition and function in different individuals. This aids in understanding the microbiome's influence on health and its role in metabolite generation.
Aging and Longevity Studies
Urolithin A is believed to possess antioxidant and anti-inflammatory properties, potentially positively impacting the aging process. Analyzing Urolithin A levels contributes to studying its potential role in maintaining mitochondrial health and autophagy, thereby exploring its relationship with aging and longevity.
Disease Prevention and Treatment Research
Urolithin A's antioxidant and anti-inflammatory characteristics make it a candidate for investigating its potential in preventing or treating neurodegenerative disorders, cardiovascular diseases, and metabolic syndrome. Analyzing Urolithin A levels and its effects on cellular processes aids in understanding its potential therapeutic applications.
Reference
- Cerdá, Begoña, et al. "Identification of urolithin A as a metabolite produced by human colon microflora from ellagic acid and related compounds." Journal of agricultural and food chemistry 53.14 (2005): 5571-5576.
For Research Use Only. Not for use in diagnostic procedures.
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