Feces Metabolomics Service

Fecal metabolomics is a new tool to explore the links between microbial composition, host phenotype and complex genetic traits. Creative Proteomics has built a metabolomic analysis platform for feces samples using high-resolution mass spectrometry. We will use the appropriate sample pretreatment protocol for your experimental purposes, significantly improving the accuracy of feces metabolite identification and quantification of metabolites.

What We Can Provide:

Untargeted metabolomics analysis:

Metabolic profile analysis
Screening for differential metabolites
Metabolic pathway analysis of differential metabolites
Discover new biomarkers

Fig 1. The workflow of untargeted metabolomics analysis

Targeted metabolomics analysis:

Our metabolomics platform provides a complete analysis of metabolites in feces, including short-chain fatty acids, amino acids and microbial metabolites.

Mode: SRM / MRM
Analysis content: Standard curve creation. Raw data preprocessing. Absolute quantification of metabolites.
Detectable substances include but are not limited to:

Short chain fatty acids Formate Propionate Valerate Acetate Butyrate Isobutyrate Isovalerate	Amino acids Lysine Alanine Glycine Glutamate Methionine Tyrosine Phenylalanine Valine Leucine Isoleucina	Microbial metabolism TMAO Dimethylamine	Glucose metabolism Glucosa Lactate Succinate	Diet metabolism Xylose Galactose
Pyrimidine metabolism Uracil Uridine	Purine metabolism Oxypurinol Hypoxanthine Inosine 2-deoxyinosine	Others Creatine Creatinine Malonato Choline 3-phenylpropionate

Fig 2. The workflow of target metabolomics analysis

Sample Requirements

Rat feces: ≥ 15-20 mg dry weight
Mouse feces: ≥ 15-20 mg dry weight
Human feces: a single histology of 200 mg or more is recommended.

Collection of stool samples in a sterile stool collector or other sterile utensils is required. Care should be taken to label the sample immediately and store it at low temperature, trying not to expose it to air for too long to avoid contamination and degradation. Before freezing the collected fresh stool, it is recommended to mix the sample with a sterile stick.

Deliverables

Experimental procedure
Parameters of liquid chromatography / gas chromatography and MS
MS raw data files and MS data quality checks
Bioinformatics analysis report

Turnaround time: 1-4 weeks

Bioinformatics Analysis

Standard analysis	1. Data quality control (QC correlation analysis, PCA analysis of total samples)
	2. Qualitative and quantitative results of metabolites
	3. Differential metabolite screening (PCA, PLS-DA dimensionality reduction treatment, volcano map)
	4. Differential metabolite analysis: Venn diagram, cluster heat map, metabolite correlation analysis, Z-score analysis, pathway analysis, ROC analysis
Personalized analysis	Weighted gene co-expression network analysis (WGCNA)
Personalized analysis	One way ANOVA

Creative Proteomics provides metabolomics services for feces samples, and will deliver accurate and detailed data and analysis reports to you. If you want to detect other metabolites but have not found them, you can tell us through the inquiry form, and our technicians will communicate with you.

For Research Use Only. Not for use in diagnostic procedures.