Sebum Metabolomics Service

Why Choose Sebum Metabolomics?

Sebum metabolomics offers a pragmatic and insightful approach to understanding skin health and physiology. By delving into the intricate biochemical composition of sebum, this methodology provides a nuanced perspective on lipid metabolism and its implications for various dermatological conditions. The choice of sebum metabolomics is rooted in its capacity to unveil specific metabolic signatures associated with skin disorders, offering targeted insights for diagnostic and therapeutic advancements. Furthermore, the ability to identify and quantify diverse sebum components facilitates a comprehensive understanding of individualized skin profiles, enabling personalized interventions for enhanced skincare.

Harnessing the excellence of Creative Proteomics' sebum metabolomics service unveils specific molecular markers intricately associated with the intricate complexities of skin conditions. Through a profound exploration of the composition of sebum metabolites, we have the capability to formulate bespoke therapeutic solutions and personalized skincare regimens. With our cutting-edge technological platforms, our service delivers comprehensive and precise detection of sebum metabolites, propelling breakthroughs in dermatological research. Creative Proteomics stands as your strategic partner in unraveling the intricate molecular nuances of skin physiology.

Sebum Metabolomics Analysis Project in Creative Proteomics

Targeted Metabolite Profiling:

• Quantitative analysis of specific metabolites involved in skin metabolism.
• Precise measurement of key components in sebum, such as lipids, fatty acids, and cholesterol.
• Utilization of state-of-the-art mass spectrometry techniques for accurate quantification.

Untargeted Metabolomics:

• Comprehensive analysis of a wide range of metabolites in sebum samples.
• Identification of metabolite patterns associated with different skin conditions or external influences.
• High-resolution mass spectrometry for unbiased profiling and discovery.

Isotope Tracing Studies:

• Investigation of metabolic fluxes in skin using stable isotope labeling.
• Measurement of isotopic enrichment in metabolites to understand skin metabolic pathways.
• Insights into sebum composition, lipid metabolism, and more.

Metabolic Pathway Analysis:

• Mapping of metabolic pathways in sebum, identifying key intermediates and regulatory metabolites.
• Integration of data to provide a holistic view of metabolic networks within the skin.

Data Analysis and Interpretation:

• Expert data processing and statistical analysis of metabolomics data.
• Identification of significant metabolite changes and pathway alterations.
• Generation of comprehensive reports with actionable insights for skincare research.

Sebum Metabolites Assay Platforms

Liquid Chromatography-Mass Spectrometry (LC-MS): The Agilent 1290 Infinity II LC System coupled with Agilent 6545 Q-TOF Mass Spectrometer facilitates accurate quantification and profiling of polar and non-polar metabolites in sebum samples. The high resolution and mass accuracy of the Agilent 6545 Q-TOF Mass Spectrometer ensure a detailed and comprehensive analysis of sebum components.

Gas Chromatography-Mass Spectrometry (GC-MS): The Thermo Scientific TRACE™ 1310 Gas Chromatograph coupled with Thermo Scientific Q Exactive™ GC Orbitrap™ Mass Spectrometer allows the quantitative measurement of volatile and semi-volatile metabolites in sebum. This platform provides detailed insights into metabolites involved in energy metabolism and offers a comprehensive view of sebum composition.

Stable Isotope Tracing Mass Spectrometry: For GC-MS, the Agilent 7890B Gas Chromatograph coupled with Agilent 7200 Q-TOF Mass Spectrometer, and for LC-MS, the Waters ACQUITY UPLC System coupled with Waters Xevo G2-XS Q-TOF Mass Spectrometer are instrumental in investigating metabolic fluxes in sebum using stable isotope labeling. These advanced instruments provide insights into substrate utilization and metabolic pathways.

Sebum Metabolomics Metabolites that can be analyzed

Lipids

Amino Acids

Sugars

Proteins

Sample Requirements of Sebum Metabolomics

Sample Types

• Whole Sebum: Complete sebum samples, including lipids, proteins, and metabolites, provide a holistic overview of skin health.
• Sebocyte-Derived Sebum: Focused specifically on sebocyte-secreted components, this sample type offers insights into cellular metabolism.
• Surface Lipid Extracts: Isolating lipids from sebum provides a detailed analysis of lipidomic profiles.
• Sebum in Different Skin Conditions: Collecting samples from individuals with various skin conditions (e.g., oily skin, dry skin, acne-prone skin) allows for targeted investigations into specific metabolic changes.

Recommended Sample Volumes:

Sample Collection and Preservation:

• Clean Collection Tools: Ensure that tools used for sample collection (e.g., swabs, spatulas) are clean and free from contaminants.
• Avoid External Contaminants: Minimize the risk of external contaminants by collecting samples in a controlled environment.
• Proper Storage: Store collected sebum samples in sterile containers or tubes to prevent degradation or contamination. Seal tightly to avoid exposure to air.
• Cold Storage: Whenever possible, store samples at a low temperature (e.g., -80°C) to maintain metabolite stability.
• Record Metadata: Accurate record-keeping of sample metadata, including donor characteristics and environmental conditions, enhances the interpretability of metabolomic data.