Puerarin Analysis Service

Overview

Puerarin is an isoflavone compound extracted from the dried roots of the legume pueraria lobata or pueraria lobata, which can improve myocardial metabolism and vascular microcirculation, and dilate coronary blood vessels. Puerarin has low toxicity, clear and extensive pharmacological effects. It has the functions of improving immunity, dilating blood vessels, lowering blood pressure, protecting myocardial cells, anti-tumor, anti-aging, promoting blood circulation, producing body fluid and anti-platelet aggregation. It is widely used in a variety of applications of pharmaceutical products. The UPLC-MS/MS method has the characteristics of strong specificity and high sensitivity. It can make the anti-interference ability strong through the correspondence between the characteristic parent ions and product ions of different compounds, and realize the functions of separation and identification, which provides a reference for the quality control and evaluation of puerarin.

Figure 1. Chemical structures of puerarin and its metabolites. (Zhang 2019)

Applications of Puerarin Analysis

The content analysis and application of puerarin
Quality control of active molecules in drugs
Development of medicinal value and evaluation of efficacy
Improve the understanding of the mechanism of puerarin's biological activity

Advantages of Our Puerarin Analysis Service

Highly selective determination of puerarin content using first-class UPLC-MS/MS platform
Provide innovative technical tools and experienced scientific research team
Meet the testing needs of multiple types of complex samples
Fast turnaround time and low price
Provide one-stop service and high-quality service level

Service Workflow

Our operation technology process adopts the method of switching between positive and negative ions. The acquisition mode selects MRM to scan the sample to be tested, and the most abundant product ion is used as the quantitative ion, which ensures the specificity and accuracy of the method.

Figure 2. Puerarin analysis service workflow.

Detection method: quantitative analysis by external standard method

Mobile phase: acetonitrile-4mmol/l ammonium formate aqueous solution

Elution method: isocratic elution

Volume flow: 1ml/min

Average sample recovery rate: 98.75%-100.86%

Injection volume: 4ul

Reaction mode: positive ion multiple reaction monitoring (MRM) mode

Analysis content:

Standard curve drawing
Selection of chromatographic conditions
Precision, repeatability and stability test
Sample recovery test
Chromatogram and mass spectrum raw image and data collection
Determination of Puerarin

Sample Requirements

1. The samples from the separated roots, stems and other tissues are quickly rinsed with sterile water, and the absorbent paper is immediately placed in the liquid after absorbing the excess water. Quickly freeze in nitrogen for more than 2 minutes, then transfer to -80°C for storage, avoid repeated freezing and thawing.

2. For each sample, take no less than 3 g for fresh samples, no less than 1 g for dry samples. At least three biological replicates in each group, prepare a backup. The measured sample will not be returned, please keep a backup.

Delivery

Comprehensive experimental procedure
Sample pretreatment analysis
UPLC-MS/MS chromatogram and mass spectrum and data analysis report
Quantitative determination of puerarin
Customized analysis report

UPLC-MS/MS can realize highly sensitive and selective determination and analysis of the content of puerarin, which can meet the determination and analysis of actual samples with low content of puerarin. The simple and fast operation steps can greatly shorten the analysis cycle. Creative Proteomics has rich biological resources and a first-class UPLC-MS/MS determination platform, and we are dedicated to providing you with quality services.

Reference

Jung HR, Kim SJ, Ham SH, et al. Simultaneous determination of puerarin and its active metabolite in human plasma by UPLC-MS/MS: application to a pharmacokinetic study. Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences. 2014;971:64-71.

For Research Use Only. Not for use in diagnostic procedures.