Alkaloids Analysis Service

What are Alkaloids?

Alkaloids are naturally occurring substances characterized by the presence of a heterocyclic ring structure that contains at least one nitrogen atom. In fact, they are ubiquitous in nature and can be found in an assortment of plants, fungi, and animals. The vast pharmacological and toxicological effects that alkaloids possess have garnered them much attention from both traditional healers and modern researchers alike. Prominent examples of alkaloids include caffeine, nicotine, morphine, and quinine. Despite the longstanding use of alkaloids in traditional medicine, their therapeutic potential continues to be a topic of great interest in contemporary scientific inquiry.

Alkaloids Analysis: Why is it Important?

It is crucial to analyze alkaloids to fully comprehend their properties and potential applications. Alkaloids can display various biological activities, including anti-inflammatory, anti-cancer, and anti-microbial effects. Nevertheless, their complex structures and diverse properties make it arduous to identify and characterize them. Commonly employed analytical techniques, such as chromatography, mass spectrometry, and nuclear magnetic resonance (NMR) spectroscopy, are necessary to identify and quantify alkaloids in complex biological matrices. However, these techniques require specialized expertise and equipment, which can be challenging for many research labs to acquire and maintain.

Alkaloids Analysis in Creative Proteomics

Creative Proteomics' team of experts has extensive experience in the identification and characterization of alkaloids, and we utilize state-of-the-art equipment and techniques to provide accurate and reliable results.

One of our key strengths is their ability to handle complex biological matrices. Alkaloids are often found in complex mixtures, such as plant extracts or biological fluids, which makes their identification and quantification challenging. We have developed specialized sample preparation protocols and analytical methods that can detect and quantify alkaloids in these complex matrices with high accuracy and precision.

We offer a wide range of services that can benefit researchers in the field of alkaloid analysis. These services include

• Alkaloid Identification: Identification and characterization of alkaloids using a variety of mass spectrometry-based techniques, including liquid chromatography-mass spectrometry (LC-MS) and gas chromatography-mass spectrometry (GC-MS).
• Alkaloid Quantification: Targeted or non-targeted mass spectrometry-based methods for quantification of alkaloids in complex biological matrices.
• Alkaloid Analysis: Generate comprehensive analyses of alkaloids in biological samples, providing insights into alkaloid distribution and potential biological activity.
• Alkaloid purification: Isolation and purification of alkaloids from complex mixtures to facilitate their further characterization and potential therapeutic use.

List of Detectable Alkaloids in Creative Proteomics

※The project items are constantly being updated, not only those listed, please contact our staff via email to get more latest information and related information.

Technical features

Several platforms

• GC- MS
• LC-MS/MS
• NMR

Qualitative and accurate

Absolute quantification

Strict quality control

The technical route of targeted metabolomics of alkaloids

Service process

Consultation for details by email or phone

Signing a contract

Fill in the information sheet

Sample submission

Detection and analysis

Output experiment report

Delivery and after-sales Q&A

Delivery standard

• Experimental procedure
• Parameters of HPLC and MS
• Raw data, chromatograms and mass spectra
• Metabolites quantification data
• Custom analysis report

Project cycle

It takes about 1-4 weeks.

Creative has leading technology, first-class R&D and production equipment, and has established a complete metabolome service system. Our professional team has many years of experience in metabolomics, bioinformatics, and statistics, and can help you plan, conduct and report metabolomics research, so that your upstream and downstream experiments can be seamlessly connected. We can provide a wide range of target metabolomics services, including target metabolic pathways, target metabolite analysis, etc. You can also perform personalized customization services at the same time during the data analysis stage. If you have any questions or specific requirements, please feel free to contact us.

References

1. Jillian M, Hagel, Rupasri, et al. Metabolome analysis of 20 taxonomically related benzylisoquinoline alkaloid-producing plants.. BMC plant biology, 2015.
2. Yida, Zhang, Xiqing, et al. Metabolomics of clinical poisoning by aconitum alkaloids using derivatization LC-MS. Frontiers in pharmacology, 2019.
3. Shimizu T, Watanabe M, Fernie A R, et al. Targeted LC-MS analysis for plant secondary metabolites[M].Plant Metabolomics. 2018.