Metabolomics Creative Proteomics

Creative Proteomics Metabolomics

Monocrotaline Analysis Service

Monocrotaline Analysis Service

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About monocrotaline

Monocrotaline is an alkaloid extracted from the seed of the Crotalaria spectabilis plant in Legumes.

The chemical formula of monocrotaline is C16H23NO6, and the CAS number is 315-22-0.

Monocrotaline a natural ligand exhibits dose-dependent cytotoxicity with potent antineoplastic activity.

MS-based technical means can realize the qualitative and quantitative analysis of monocrotaline.

Molecular structure of monocrotalineMolecular structure of monocrotaline

Workflow of targeted metabolomics service of monocrotaline

Design experiments and sample processing

Standard preparation and mass spectrometry detection, and make standard curve

Extraction of metabolites in samples and mass spectrometry detection, and raw data preprocessing

Qualitative and quantitative of target metabolites

Bioinformatics analysis (PCA analysis, cluster analysis, difference analysis, etc.)

Workflow of targeted metabolomics service of monocrotaline

Sample requirements

Sample demand
Fresh and clean plant / animal samples > 1 g, freeze-dried sample > 0.5 g
Animal body fluid samples > 200 µl
Stool, etc. > 100 mg
Sample mixed and repeat
Each sample was mixed from more than 6 same condition samples, and quickly put into liquid nitrogen.
※ The same condition refers to the same period, basically the same phenotype, and the same part.
It is recommended that prepare more than 6 biological replicates.
Storage and transportation
Quick-frozen preservation with liquid nitrogen can minimize the leakage time of plant samples at room temperature, and store it at -80℃.
Dry ice transportation (about 3~4kg dry ice is consumed every day, please use sufficient dry ice for transportation).

Service process

Service process

Delivery standard

  • Experimental procedure
  • Parameters of HPLC and MS
  • Raw data, chromatograms and mass spectra
  • Metabolites quantification data
  • Custom analysis report

Project cycle

A standard experiment and analysis process takes about 1-4 weeks.

Creative Proteomics offers several approaches to plant metabolomics studies, delivers precise and detailed data and analysis report. We can also customize the methods or establish new methods together with our collaborators, so they are fit-for-purpose and meet your specific needs. If you have any questions or specific requirements, please feel free to contact us.

References

  1. Yao Y, Wang G B, Man S L, et al. Toxicological advances of traditional medicine in 2019. Traditional Medical Research (TMR), 2020, 005(002): P.83-89.
  2. Kurek J. Cytotoxic Colchicine Alkaloids: From Plants to Drugs. Cytotoxicity. 2018.
  3. Luca V D, Salim V, Atsumi S M, et al. Mining the Biodiversity of Plants: A Revolution in the Making. Science, 2012.
For Research Use Only. Not for use in diagnostic procedures.

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