Metabolomics Creative Proteomics

Creative Proteomics Metabolomics

Colchicine Analysis Service

Colchicine Analysis Service

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Creative Proteomics' targeted metabolomics services target specific metabolites and pathways of interest, which can accurately monitor dynamic metabolic processes, reveal related metabolic mechanisms and verify potential metabolic biomarkers, so as to guide your research with reliable and accurate measurement results.

About colchicine

Colchicine, an alkaloid, was originally extracted from colchicine in the Liliaceae plant. Its IUPAC name is N-[(7S)-1,2,3,10-Tetramethoxy-9-oxo-5,6,7,9-tetrahydrobenzo[a]heptalen-7-yl] acetamide.

The chemical formula of colchicine is C22H25NO6, and the CAS number is 64-86-8.

Colchicine can inhibit mitosis, destroy the spindle, and make the chromosomes stagnate in the metaphase. Therefore, it is widely used in cytology, genetics research and plant breeding.

Colchicine can inhibit the growth of cancer cells. It is used clinically to treat cancer, gout and other diseases.

MS-based technical means can realize the qualitative and quantitative analysis of colchicine.

Molecular structure of colchicineMolecular structure of colchicine

Workflow of targeted metabolomics service of colchicine

With years of technical accumulation, Creative Proteomics adopts an advanced platform and cooperates with isotope standards for qualitative and quantitative analysis of related substances.

Workflow of targeted metabolomics service of colchicine

Sample requirements

Sample demand
Fresh plant / animal tissue > 200 mg
Number of cells or bacteria > 107, supernatant > 2 ml
Animal body fluid samples > 200 µl.
For other sample types, please consult technical support or sales.
Sample mixed and repeat
In order to ensure the accuracy of the samples and reduce systematic errors during sampling, it is necessary to select more than 3 materials with the same condition for each sample.
※ The same condition refers to the same period, basically the same phenotype, and the same part.
It is recommended that prepare more than 6 biological replicates.
Storage and transportation
Quick-frozen preservation with liquid nitrogen can minimize the leakage time of plant samples at room temperature, and store it at -80℃.
Dry ice transportation (about 3~4kg dry ice is consumed every day, please use sufficient dry ice for transportation).

Delivery standard

  • Experimental procedure
  • Parameters of HPLC and MS
  • Raw data, chromatograms and mass spectra
  • Metabolites quantification data
  • Custom analysis report

Project cycle

The entire standard process takes about 1-4 weeks.

References

  1. Kurek J. Cytotoxic Colchicine Alkaloids: From Plants to Drugs. Cytotoxicity. 2018.
  2. Luca V D, Salim V, Atsumi S M, et al. Mining the Biodiversity of Plants: A Revolution in the Making. Science, 2012.
For Research Use Only. Not for use in diagnostic procedures.

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